Eugenia uniflora L. Essential Oil as a Potential Anti-Leishmania Agent: Effects on Leishmania amazonensis and Possible Mechanisms of Action
In: Evidence-based Complementary and Alternative Medicine : eCAM, Jg. 2013 (2013-02-01)
Online
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Zugriff:
Eugenia unifloraL. is a member of the Myrtaceae family and is commonly known as Brazilian cherry tree. In this study, we evaluated the chemical composition ofEugenia unifloraL. essential oil (EuEO) by using gas chromatography-mass spectrometry (GC-MS) and assessed its anti-Leishmaniaactivity. We also explored the potential mechanisms of action and cytotoxicity of EuEO. Thirty-two compounds were identified, which constituted 92.65% of the total oil composition. The most abundant components were sesquiterpenes (91.92%), with curzerene (47.3%),γ-elemene (14.25%), andtrans-β-elemenone (10.4%) being the major constituents. The bioactivity shown by EuEO against promastigotes (IC50, 3.04 μg·mL−1) and amastigotes (IC50, 1.92 μg·mL−1) suggested significant anti-Leishmaniaactivity. In the cytotoxicity determination, EuEO was 20 times more toxic to amastigotes than to macrophages. Hemolytic activity was 63.22% at the highest concentration tested (400 μg·mL−1); however, there appeared to be no toxicity at 50 μg·mL−1. While the data show that EuEO activity is not mediated by nitric oxide production, they do suggest that macrophage activation may be involved in EuEO anti-Leishmaniaactivity, as evidenced by increases in both the phagocytic capacity and the lysosomal activity. More studies are needed to determinein vivoactivity as well as additional mechanisms of the anti-Leishmaniaactivity.
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Eugenia uniflora L. Essential Oil as a Potential Anti-Leishmania Agent: Effects on Leishmania amazonensis and Possible Mechanisms of Action
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Autor/in / Beteiligte Person: | Fernando Aécio de Amorim Carvalho ; Sabrina Maria Portela Carneiro ; Layane Valéria Amorim ; Clarice Noleto Dias ; José Guilherme S. Maia ; Eloisa Helena A. Andrade ; Klinger Antonio da Franca Rodrigues ; Jamylla Mirck Guerra de Oliveira ; Denise Fernandes Coutinho Moraes |
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Zeitschrift: | Evidence-based Complementary and Alternative Medicine : eCAM, Jg. 2013 (2013-02-01) |
Veröffentlichung: | Hindawi Publishing Corporation, 2013 |
Medientyp: | unknown |
ISSN: | 1741-4288 (print) ; 1741-427X (print) |
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