A PEST-Like Sequence in Listeriolysin O Essential forListeria monocytogenesPathogenicity
In: Science, Jg. 290 (2000-11-03), S. 992-995
Online
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Zugriff:
Establishment and maintenance of an intracellular niche are critical to the success of an intracellular pathogen. Here, the pore-forming protein listeriolysin O (LLO), secreted byListeria monocytogenes, was shown to contain a PEST-like sequence (P, Pro; E, Glu; S, Ser; T, Thr) that is essential for the virulence and intracellular compartmentalization of this pathogen. Mutants lacking the PEST-like sequence entered the host cytosol but subsequently permeabilized and killed the host cell. LLO lacking the PEST-like sequence accumulated in the host-cell cytosol, suggesting that this sequence targets LLO for degradation. Transfer of the sequence to perfringolysin O transformed this toxic cytolysin into a nontoxic derivative that facilitated intracellular growth.
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A PEST-Like Sequence in Listeriolysin O Essential forListeria monocytogenesPathogenicity
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Autor/in / Beteiligte Person: | Decatur, Amy L. ; Portnoy, Daniel A. |
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Zeitschrift: | Science, Jg. 290 (2000-11-03), S. 992-995 |
Veröffentlichung: | American Association for the Advancement of Science (AAAS), 2000 |
Medientyp: | unknown |
ISSN: | 1095-9203 (print) ; 0036-8075 (print) |
DOI: | 10.1126/science.290.5493.992 |
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