Essential requirement of an invariant V alpha 14 T cell antigen receptor expression in the development of natural killer T cells
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 93 (1996-10-01), Heft 20
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Zugriff:
NK1.1+ T [natural killer (NK) T] cells express an invariant T cell antigen receptor alpha chain (TCR alpha) encoded by V alpha 14 and J alpha 281 segments in association with a limited number of V betas, predominantly V beta 8.2. Expression of the invariant V alpha 14/J alpha 281, but not V alpha 1, TCR in transgenic mice lacking endogenous TCR alpha expression blocks the development of conventional T alpha beta cells and leads to the preferential development of V alpha 14 NK T cells, suggesting a prerequisite role of invariant V alpha 14 TCR in NK T cell development. In V beta 8.2 but not B beta 3 transgenic mice, two NK T cells with different CD3 epsilon expressions, CD3 epsilon(dim) and CD3 epsilon(high), can be identified. CD3 epsilon(high) NK T cells express surface V alpha 14/V beta 8 TCR, indicating a mature cell type, whereas CD3 epsilon(dim) NK T cells express V beta 8 without V alpha 14 TCR and no significant CD3 epsilon expression (CD3 epsilon(dim)) on the cell surface. However, the latter are positive for recombination activating gene (RAG-1 and RAG-2) mRNA, which are only expressed in the precursor or immature T cell lineage, and also possess CD3 epsilon mRNA in their cytoplasm, suggesting that CD3 epsilon(dim) NK T cells are the precursor of V alpha 14 NK T cells.
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Essential requirement of an invariant V alpha 14 T cell antigen receptor expression in the development of natural killer T cells
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Autor/in / Beteiligte Person: | Tokuhisa, Takeshi ; Taniguchi, Masaru ; Koyasu, Shigeo ; Cui, Junqing ; Perkes, Alysia ; Kawano, Tetsu ; Masuda, Kimio ; Makino, Yasuhiko ; Kondo, Eisuke ; Koseki, Haruhiko ; Sato, Hiroshi |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 93 (1996-10-01), Heft 20 |
Veröffentlichung: | 1996 |
Medientyp: | unknown |
ISSN: | 0027-8424 (print) |
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