COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines
In: Nucleic Acids Research, Jg. 40 (2011-11-18), S. D957- (7S.)
Online
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Zugriff:
Genome-wide pooled shRNA screens enable global identification of the genes essential for cancer cell survival and proliferation and provide a ‘functional genetic’ map of human cancer to complement genomic studies. Using a lentiviral shRNA library targeting approximately 16 000 human genes and a newly developed scoring approach, we identified essential gene profiles in more than 70 breast, pancreatic and ovarian cancer cell lines. We developed a web-accessible database system for capturing information from each step in our standardized screening pipeline and a gene-centric search tool for exploring shRNA activities within a given cell line or across multiple cell lines. The database consists of a laboratory information and management system for tracking each step of a pooled shRNA screen as well as a web interface for querying and visualization of shRNA and gene-level performance across multiple cancer cell lines. COLT-Cancer Version 1.0 is currently accessible at http://colt.ccbr.utoronto.ca/cancer.
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COLT-Cancer: functional genetic screening resource for essential genes in human cancer cell lines
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| Autor/in / Beteiligte Person: | Koh, Judice L. Y. ; Brown, Kevin R. ; Kasimer, Dahlia ; Moffat, Jason ; Sayad, Azin ; Ketela, Troy |
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| Zeitschrift: | Nucleic Acids Research, Jg. 40 (2011-11-18), S. D957- (7S.) |
| Veröffentlichung: | Oxford University Press (OUP), 2011 |
| Medientyp: | unknown |
| ISSN: | 1362-4962 (print) ; 0305-1048 (print) |
| DOI: | 10.1093/nar/gkr959 |
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