Bio-Guided Fractionation Driven by In Vitro α-Amylase Inhibition Assays of Essential Oils Bearing Specialized Metabolites with Potential Hypoglycemic Activity
In: Plants, Jg. 9 (2020-09-21), Heft 1242, p 1242
Online
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Zugriff:
Type 2 diabetes mellitus (T2DM) is a metabolic disorder characterized by unpaired blood glycaemia maintenance. T2DM can be treated by inhibiting carbohydrate hydrolyzing enzymes (&alpha
amylases and &alpha
glucosidases) to decrease postprandial hyperglycemia. Acarbose and voglibose are inhibitors used in clinical practice. However, these drugs are associated with unpleasant gastrointestinal side effects. This study explores new &alpha
amylase inhibitors deriving from plant volatile specialized metabolites. Sixty-two essential oils (EOs) from different plant species and botanical families were subjected to &alpha
amylase in vitro enzymatic assay and chemically characterized using gas chromatography coupled to mass spectrometry. Several EOs were found to be potential &alpha
amylase inhibitors, and Eucalyptus radiata, Laurus nobilis, and Myristicafragrans EOs displayed inhibitory capacities comparable to that of the positive control (i.e., acarbose). A bio-guided fractionation approach was adopted to isolate and identify the active fractions/compounds of Eucalyptus radiata and Myristica fragrans EOs. The bio-guided fractionation revealed that EOs &alpha
amylase inhibitory activity is often the result of antagonist, additive, or synergistic interactions among their bioactive constituents and led to the identification of 1,8-cineole, 4-terpineol, &alpha
terpineol, &alpha
pinene, and &beta
pinene as bioactive compounds, also confirmed when they were tested singularly. These results demonstrate that EO oils are a promising source of potential &alpha
amylase inhibitors.
| Titel: |
Bio-Guided Fractionation Driven by In Vitro α-Amylase Inhibition Assays of Essential Oils Bearing Specialized Metabolites with Potential Hypoglycemic Activity
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| Autor/in / Beteiligte Person: | Marengo, Arianna ; Rubiolo, Patrizia ; Bicchi, Carlo ; Capetti, Francesca ; Sgorbini, Barbara ; Cagliero, Cecilia |
| Link: | |
| Zeitschrift: | Plants, Jg. 9 (2020-09-21), Heft 1242, p 1242 |
| Veröffentlichung: | Multidisciplinary Digital Publishing Institute, 2020 |
| Medientyp: | unknown |
| ISSN: | 2223-7747 (print) |
| DOI: | 10.3390/plants9091242 |
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