Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase
In: International Journal of Molecular Sciences, Jg. 21 (2020-05-01), Heft 10
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Zugriff:
Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as Sphingobium sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 °
C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by Sphingobium sp. YC-JY1. Escherichia coli BL21 (DE3) cells harboring pET28a-bisdAB achieved the ability to degrade BPA. The bisdB gene knockout strain YC-JY1&Delta
bisdB was unable to degrade BPA indicating that P450bisdB was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.
| Titel: |
Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase
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| Autor/in / Beteiligte Person: | Yan, Yanchun ; Thet Su Hlaing ; Eltoukhy, Adel ; Li, Xianjun ; Wang, Junhuan ; Mar Mar Aung ; May Thet Nwe ; Jia, Yang ; Lamraoui, Imane |
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| Zeitschrift: | International Journal of Molecular Sciences, Jg. 21 (2020-05-01), Heft 10 |
| Veröffentlichung: | MDPI, 2020 |
| Medientyp: | unknown |
| ISSN: | 1422-0067 (print) |
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