Compositional Analysis and Antioxidant Activities of Thymus pubescens Essential Oil from Iran
In: Combinatorial Chemistry & High Throughput Screening, Jg. 25 (2022-02-01), S. 252-258
Online
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Zugriff:
Background: The genus Thymus is one of the well-known members of the Lamiaceae, which comprises about 215 species. It is a polymorphic genus in this family. Therefore, possible differences in chemical composition among polymorphs may result in differences in their biological activities. Objective: This study was designed to specify chemical composition in order to assess antioxidant activities and to characterize active constituents of essential oil from Thymus pubescens. Methods: Essential oil was collected by hydrodistillation and was analyzed using GC-MS and GC-FID. Antioxidant activities were evaluated by ABTS radical cation scavenging and β-carotene bleaching inhibition methods. Detection and identification of antioxidant components were performed by TLC-bioautography assay using ABTS•+ reagent. Results: GC analyses indicated the presence of 39 components, accounting for 96.64% of total constituents, with thymol (38.67%), γ-terpinene (7.46%), and p-cymene (5.54%) as the main components. The IC50 values for antioxidant activities were equal to 1.15 (1.05 – 1.25) μg/mL and 0.45 (0.33 – 0.62) μg/mL for ABTS•+ scavenging and β-carotene bleaching inhibition tests, respectively. TLC-bioautograpgy of oil resulted in identification of thymol as the major antioxidant compound. Conclusion: Essential oil exhibited very potent and significant antioxidant activities correlated well with increasing dose in vitro.
Titel: |
Compositional Analysis and Antioxidant Activities of Thymus pubescens Essential Oil from Iran
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Autor/in / Beteiligte Person: | Nickavar, Bahman ; Malekitabar, Elaheh |
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Zeitschrift: | Combinatorial Chemistry & High Throughput Screening, Jg. 25 (2022-02-01), S. 252-258 |
Veröffentlichung: | Bentham Science Publishers Ltd., 2022 |
Medientyp: | unknown |
ISSN: | 1386-2073 (print) |
DOI: | 10.2174/1386207323666201230091042 |
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