Fingerprinting, Antimicrobial, Antioxidant, Anticancer, Cyclooxygenase and Metabolic Enzymes Inhibitory Characteristic Evaluations of Stachys viticina Boiss. Essential Oil
In: Molecules, Jg. 24 (2019-10-28), Heft 21, p 3880
Online
unknown
Zugriff:
The present study aimed to identify the chemical constituents and to assess the in-vitro, antimicrobial, anticancer, antioxidant, metabolic enzymes and cyclooxygenase (COX) inhibitory properties of essential oil (EO) of Stachys viticina Boiss. leaves. The S. viticina EO was isolated and identified using microwave-ultrasonic and GC-MS techniques, respectively. Fifty-two compounds were identified, of which endo-borneol was the major component, followed by eucalyptol and epizonarene. The EO was evaluated against a panel of in-vitro bioassays. The EO displayed antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Epidermophyton floccosum, with MIC values of 0.039, 0.078 and 0.78 mg/mL, respectively. The EO exhibited cytotoxicity against HeLa (cervical adenocarcinoma) and Colo-205 (colon) cancer cell lines with percentages of inhibition of 95% and 90%, for EO concentrations of 1.25 and 0.5 mg/mL, respectively. Furthermore, it showed metabolic enzyme (&alpha
amylase, &alpha
glucosidase, and lipase) inhibitory (IC50 = 45.22 ±
1.1, 63.09 ±
0.26, 501.18 ±
0.38 µ
g/mL, respectively) and antioxidant activity, with an IC50 value of 19.95 ±
2.08 µ
g/mL. Moreover, the S. viticina EO showed high cyclooxygenase inhibitory activity against COX-1 and COX-2 with IC50 values of 0.25 and 0.5 µ
g/mL, respectively, similar to those of the positive control (the NSAID etodolac). Outcomes amassed from this investigation illustrate that S. viticina EO represents a rich source of pharmacologically active molecules which can be further validated and explored clinically for its therapeutic potential and for the development and design of new natural therapeutic preparations.
Titel: |
Fingerprinting, Antimicrobial, Antioxidant, Anticancer, Cyclooxygenase and Metabolic Enzymes Inhibitory Characteristic Evaluations of Stachys viticina Boiss. Essential Oil
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Autor/in / Beteiligte Person: | Jaradat, Nidal ; Al-Maharik, Nawaf |
Link: | |
Zeitschrift: | Molecules, Jg. 24 (2019-10-28), Heft 21, p 3880 |
Veröffentlichung: | Multidisciplinary Digital Publishing Institute, 2019 |
Medientyp: | unknown |
ISSN: | 1420-3049 (print) |
DOI: | 10.3390/molecules24213880 |
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