The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine
In: Viruses, Jg. 12 (2020-06-01), Heft 6
Online
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Zugriff:
African swine fever virus (ASFV) is the causative agent of the African swine fever (ASF) epizootic currently affecting pigs throughout Eurasia, causing significant economic losses in the swine industry. The virus genome encodes for more than 160 genes, of which only a few have been studied in detail. Here we describe the previously uncharacterized ASFV open reading frame (ORF) C962R, a gene encoding for a putative NTPase. RNA transcription studies using infected swine macrophages demonstrate that the C962R gene is translated as a late virus protein. A recombinant ASFV lacking the C962R gene (ASFV-G-&Delta
C962R) demonstrates in vivo that the C962R gene is non-essential, since ASFV-G-&Delta
C962R has similar replication kinetics in primary swine macrophage cell cultures when compared to parental highly virulent field isolate Georgia2007 (ASFV-G). Experimental infection of domestic pigs with ASFV-G-&Delta
C962R produced a clinical disease similar to that caused by the parental ASFV-G, confirming that deletion of the C962R gene from the ASFV genome does not impact virulence.
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The C962R ORF of African Swine Fever Strain Georgia Is Non-Essential and Not Required for Virulence in Swine
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| Autor/in / Beteiligte Person: | Silva, Ediane ; Pruitt, Sarah ; Ramirez-Medina, Elizabeth ; Zhu, James ; Velazquez-Salinas, Lauro ; Gladue, Douglas P. ; Borca, Manuel V. ; Elizabeth A Vuono ; Rai, Ayushi |
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| Zeitschrift: | Viruses, Jg. 12 (2020-06-01), Heft 6 |
| Veröffentlichung: | MDPI, 2020 |
| Medientyp: | unknown |
| ISSN: | 1999-4915 (print) |
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