Atypical ALPK2 kinase is not essential for cardiac development and function
In: American Journal of Physiology-Heart and Circulatory Physiology, Jg. 318 (2020-06-01), S. H1509- (7S.)
Online
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Zugriff:
Protein kinases play an integral role in cardiac development, function, and disease. Recent experimental and clinical data have implied that protein kinases belonging to a family of atypical α-protein kinases, including α-protein kinase 2 (ALPK2), are important for regulating cardiac development and maintaining function via regulation of WNT signaling. A recent study in zebrafish reported that loss of ALPK2 leads to severe cardiac defects; however, the relevance of ALPK2 has not been studied in a mammalian animal model. To assess the role of ALPK2 in the mammalian heart, we generated two independent global Alpk2-knockout (Alpk2-gKO) mouse lines, using CRISPR/Cas9 technology. We performed physiological and biochemical analyses of Alpk2-gKO mice to determine the functional, morphological, and molecular consequences of Alpk2 deletion at the organismal level. We found that Alpk2-gKO mice exhibited normal cardiac function and morphology up to one year of age. Moreover, we did not observe altered WNT signaling in neonatal Alpk2-gKO mouse hearts. In conclusion, Alpk2 is dispensable for cardiac development and function in the murine model. Our results suggest that Alpk2 is a rapidly evolving gene that lost its essential cardiac functions in mammals. NEW & NOTEWORTHY Several studies indicated the importance of ALPK2 for cardiac function and development. A recent study in zebrafish report that loss of ALPK2 leads to severe cardiac defects. In contrast, murine Alpk2-gKO models developed in this work display no overt cardiac phenotype. Our results suggest ALPK2, as a rapidly evolving gene, lost its essential cardiac functions in mammals.
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Atypical ALPK2 kinase is not essential for cardiac development and function
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Autor/in / Beteiligte Person: | Matthew Daniel Yu ; Chen, Ju ; Zhang, Lunfeng ; Spinozzi, Simone ; Feng, Wei ; Gu, Yusu ; Huang, Serena ; Trexler, Christa ; Bogomolovas, Julijus |
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Zeitschrift: | American Journal of Physiology-Heart and Circulatory Physiology, Jg. 318 (2020-06-01), S. H1509- (7S.) |
Veröffentlichung: | American Physiological Society, 2020 |
Medientyp: | unknown |
ISSN: | 1522-1539 (print) ; 0363-6135 (print) |
DOI: | 10.1152/ajpheart.00249.2020 |
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