Potent immunogenicity of the B subunits of Escherichia coli heat-labile enterotoxin: receptor binding is essential and induces differential modulation of lymphocyte subsets
In: Proceedings of the National Academy of Sciences, Jg. 93 (1996-01-09), S. 226-230
Online
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Zugriff:
The importance of receptor binding in the potent immunogenicity of Escherichia coli heat-labile enterotoxin B subunit (EtxB) was tested by comparing its immunogical properties with those of a receptor binding mutant, EtxB(G33D). Subcutaneous immunization of EtxB(G33D) resulted in 160-fold reduction in antibody titer compared with wild-type EtxB, whereas its oral delivery failed to provoke any detectable secretory or serum anti-B subunit responses. Moreover, the two proteins induced strikingly different effects on lymphocyte cultures in vitro. EtxB, in comparison with EtxB(G33D), caused an increase in the proportion of B cells, many of which were activated (CD25+); the complete depletion of CD8+ T cells; an increase in the activation of CD4+ T cells; and an increase in interleukin 2 and a decrease in interferon gamma. These data indicate that EtxB exerts profound effects on immune cells, suggesting that its potent immunogenicity is dependent not only on efficient receptor-mediated uptake, but also on direct receptor-mediated immunomodulation of lymphocyte subsets.
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Potent immunogenicity of the B subunits of Escherichia coli heat-labile enterotoxin: receptor binding is essential and induces differential modulation of lymphocyte subsets
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Autor/in / Beteiligte Person: | Eaglestone, Simon S. ; Nashar, Toufic O. ; Hirst, Timothy R. ; Webb, Helen M. ; Williams, Neil A. |
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Zeitschrift: | Proceedings of the National Academy of Sciences, Jg. 93 (1996-01-09), S. 226-230 |
Veröffentlichung: | Proceedings of the National Academy of Sciences, 1996 |
Medientyp: | unknown |
ISSN: | 1091-6490 (print) ; 0027-8424 (print) |
DOI: | 10.1073/pnas.93.1.226 |
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