Evolutionarily conserved E(y)2/Sus1 protein is essential for the barrier activity of Su(Hw)-dependent insulators in Drosophila
In: Molecular cell, Jg. 27 (2006-10-12), Heft 2
Online
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Zugriff:
Summary Chromatin insulators affect interactions between promoters and enhancers/silencers and function as barriers for spreading of repressive chromatin. The Su(Hw) protein is responsible for activity of the best-studied Drosophila insulators. Here we demonstrate that an evolutionarily conserved protein, E(y)2/Sus1, is recruited to the Su(Hw) insulators via binding to the zinc-finger domain of Su(Hw). Partial inactivation of E(y)2 in a weak mutation, e(y)2 u1 , impairs only the barrier, but not the enhancer-blocking, activity of the Su(Hw) insulators. Whereas neither su(Hw) − nor e(y)2 u1 affects fly viability, their combination proves lethal, testifying to functional interaction between Su(Hw) and E(y)2 in vivo. Apparently, different domains of Su(Hw) recruit proteins responsible for enhancer-blocking and for the barrier activity.
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Evolutionarily conserved E(y)2/Sus1 protein is essential for the barrier activity of Su(Hw)-dependent insulators in Drosophila
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Autor/in / Beteiligte Person: | Kurshakova, M. M. ; Maksimenko, Oksana ; Georgiev, Pavel ; Golovnin, Anton ; Krasnov, Aleksey N. ; Pulina, Maria ; Georgieva, Sofia G. |
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Zeitschrift: | Molecular cell, Jg. 27 (2006-10-12), Heft 2 |
Veröffentlichung: | 2006 |
Medientyp: | unknown |
ISSN: | 1097-2765 (print) |
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