Towards gas chromatography–mass spectrometry coupling protocols for both identifying and quantification essential oils of Thymus capitatus Hoff et Link
In: Journal of Chromatography A, Jg. 1064 (2005), S. 129-134
Online
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Zugriff:
Essential oil of Thymus capitatus Hoff et Link is analysed by using four techniques: GC/pyrolyse/MS, GC/FID, electronic impact GC/MS (quadripole), and GC/MS (ion trap). Both major and trace components are analysed. The GC/pyrolyse/MS coupling provides reference to the exact mass compositions without any need of the previously purified references, neither for major or trace components. The comparison between this reference analysis and GC/FID shows that the FID response coefficients may vary by a mean 7% from one component to another. As it was expected, quadripole or ion trap response coefficients vary to a much greater extent (a mean 25%), although the two MS techniques response coefficients are first order consistent. We conclude that GC/MS coupling could be used not only as it is usual for reliable identifications, but also for a complete quantitative routine analysis of essential oils. Expected precision could be very similar to GC/FID precision provided correcting species by species the MS analysis by a mean value of the response coefficient measured for the MS 70 eV electronic impact ionisation technologies. The GC/pyrolyse/MS coupling is proposed as a relevant tool for analysing reference samples containing trace natural species that could not be purified.
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Towards gas chromatography–mass spectrometry coupling protocols for both identifying and quantification essential oils of Thymus capitatus Hoff et Link
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Autor/in / Beteiligte Person: | Abderrabba, Manef ; Planche, Henri ; Hedhili, Lassaad ; Romdhane, Mehrez |
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Zeitschrift: | Journal of Chromatography A, Jg. 1064 (2005), S. 129-134 |
Veröffentlichung: | Elsevier BV, 2005 |
Medientyp: | unknown |
ISSN: | 0021-9673 (print) |
DOI: | 10.1016/j.chroma.2004.11.095 |
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