Antitumor Effect of the Essential Oil from the Leaves of Croton matourensis Aubl. (Euphorbiaceae)
In: Molecules, Jg. 23 (2018-11-01), Heft 11
Online
unknown
Zugriff:
Croton matourensis Aubl. (synonym Croton lanjouwensis Jabl.), popularly known as &ldquo
orelha de burro&rdquo
&ldquo
maravuvuia&rdquo
and/or &ldquo
sangrad&rsquo
á
gua&rdquo
is a medicinal plant used in Brazilian folk medicine as a depurative and in the treatment of infections, fractures, and colds. In this work, we investigated the chemical composition and in vitro cytotoxic and in vivo antitumor effects of the essential oil (EO) from the leaves of C. matourensis collected from the Amazon rainforest. The EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC&ndash
MS) and gas chromatography with flame ionization detection (GC&ndash
FID), respectively. In vitro cytotoxicity of the EO was assessed in cancer cell lines (MCF-7, HCT116, HepG2, and HL-60) and the non-cancer cell line (MRC-5) using the Alamar blue assay. Furthermore, annexin V-FITC/PI staining and the cell cycle distribution were evaluated with EO-treated HepG2 cells by flow cytometry. In vivo efficacy of the EO (40 and 80 mg/kg/day) was demonstrated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The EO included &beta
caryophyllene, thunbergol, cembrene, p-cymene, and &beta
elemene as major constituents. The EO exhibited promising cytotoxicity and was able to cause phosphatidylserine externalization and DNA fragmentation without loss of the cell membrane integrity in HepG2 cells. In vivo tumor mass inhibition rates of the EO were 34.6% to 55.9%. Altogether, these data indicate the anticancer potential effect of C. matourensis.
Titel: |
Antitumor Effect of the Essential Oil from the Leaves of Croton matourensis Aubl. (Euphorbiaceae)
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Autor/in / Beteiligte Person: | Talita A. da Anunciação ; Rafaela G Alves ; Emilly J. S. P. de Lima ; Bezerra, Daniel P. ; Milena Botelho Pereira Soares ; Cardozo, Nállarett M. D. ; Gigliola M A D Elia ; Felipe M. A. da Silva ; Koolen, Hector H. F. ; Luciano de S. Santos ; Costa, Emmanoel V. ; Silva, Valdenizia R. |
Link: | |
Zeitschrift: | Molecules, Jg. 23 (2018-11-01), Heft 11 |
Veröffentlichung: | MDPI, 2018 |
Medientyp: | unknown |
ISSN: | 1420-3049 (print) |
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