Capture of a third Mg 2+ is essential for catalyzing DNA synthesis
In: Science, Jg. 352 (2016-06-10), S. 1334-1337
Online
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Zugriff:
A hit-and-run metal ion DNA polymerase is an enzyme that uses existing DNA as a template to build new DNA by adding new nucleotides to the end of the newly forming daughter strand. The enzyme mechanism that catalyzes formation of a phosphodiester bond is known to require two Mg 2+ ions, and recent crystal structures have shown that a third metal ion is present after bond formation. Gao et al. used time-resolved crystallography to visualize bond formation. The enzyme-substrate complex captures a third cation before bond formation occurs, and DNA synthesis cannot occur without the third metal ion. Binding of this metal ion requires thermal motion of the enzyme-substrate complex, so that catalysis is achieved by acquiring a transient cofactor. Science , this issue p. 1334
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Capture of a third Mg 2+ is essential for catalyzing DNA synthesis
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Autor/in / Beteiligte Person: | Yang, Wei ; Gao, Yang |
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Zeitschrift: | Science, Jg. 352 (2016-06-10), S. 1334-1337 |
Veröffentlichung: | American Association for the Advancement of Science (AAAS), 2016 |
Medientyp: | unknown |
ISSN: | 1095-9203 (print) ; 0036-8075 (print) |
DOI: | 10.1126/science.aad9633 |
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