Structural insights into RNase J that plays an essential role in Mycobacterium tuberculosis RNA metabolism
In: Nature Communications, Jg. 14 (2023-04-20)
Online
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Zugriff:
Ribonucleases (RNases) are responsible for RNA metabolism. RNase J, the core enzyme of the RNA degradosome, plays an essential role in global mRNA decay. Emerging evidence showed that the RNase J of Mycobacterium tuberculosis (Mtb-RNase J) could be an excellent target for treating Mtb infection. Here, crystal structures of Mtb-RNase J in apo-state and complex with the single-strand RNA reveal the conformational change upon RNA binding and hydrolysis. Mtb-RNase J forms an active homodimer through the interactions between the β-CASP and the β-lactamase domain. Knockout of RNase J slows the growth rate and changes the colony morphologies and cell length in Mycobacterium smegmatis, which is restored by RNase J complementation. Finally, RNA-seq analysis shows that the knockout strain significantly changes the expression levels of 49 genes in metabolic pathways. Thus, our current study explores the structural basis of Mtb-RNase J and might provide a promising candidate in pharmacological treatment for tuberculosis.
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Structural insights into RNase J that plays an essential role in Mycobacterium tuberculosis RNA metabolism
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Autor/in / Beteiligte Person: | Bao, Luyao ; Hu, Juan ; Zhan, Bowen ; Chi, Mingzhe ; Li, Zhengyang ; Wang, Sen ; Shan, Chan ; Zhao, Zhaozhao ; Guo, Yanchao ; Ding, Xiaoming ; Ji, Chaoneng ; Tao, Shengce ; Ni, Ting ; Zhang, Xuelian ; Zhao, Guoping ; Li, Jixi |
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Zeitschrift: | Nature Communications, Jg. 14 (2023-04-20) |
Veröffentlichung: | Springer Science and Business Media LLC, 2023 |
Medientyp: | unknown |
ISSN: | 2041-1723 (print) |
DOI: | 10.1038/s41467-023-38045-z |
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