Essential Oil Composition, Bioactive Compounds, and Antioxidant Activities in Iberis amara L

This research was carried out to assess essential oils (EOs), total phenolic content (TPC), total flavonoids (TFD), total flavonols (TFL), total chlorophyll, total carotenoids, total anthocyanins, and different antioxidant activity assays in two plant samples (leaf and bud) of Iberis amara L. The gas chromatography mass spectrophotometry (GC-MS) of the EOs and the head space (HS)-GC-MS of the methanolic leaf extracts identified 34 and 6 different compounds, respectively. The major components of the leaf EOs were carvacrol (9.4%), camphene (6.2%), р-cymene (4.3%), and eugenol (3.8%) respectively, whereas cumin aldehyde (10.4%) was the main component in the bud sample. 1-Butene, 4-isothiocyanate (50%) was identified as the main component in the HS-GC-MS analysis of leaves extract. The highest content of TPC (32.8 ± 0.7 mg GAE/gDW), TFD (28.4 ± 0.7 mg QE/gDW), TFL (11.8 ± 0.06 mg QE/gFW), and anthocyanin (0.4 ±0.02 µmol/g FW) was found in the methanolic extract of leaves. The highest antioxidant activity in the phosphomolibdate assay (628.3 ± 10 µg AAE/gFW) and the least activity in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay (IC50 = 415 ± 3.1 µg/mL) was found in leaf samples. The high ratio of monoterpene hydrocarbons and oxygenated monoterpenes of the EOs along with the high antioxidant activity propose the application of this medicinal plant for general or specific applications in food industries as a herbal plant.