Chemical Composition of Actinodaphne pilosa Essential Oil From Vietnam, Mosquito Larvicidal Activity, and Antimicrobial Activity

Leaves of Actinodaphne pilosa were collected at 2 different seasons from the Pù Hoạt Nature Reserve, Vietnam. The leaf samples were hydrodistilled to give essential oils, which were analyzed by gas chromatography (GC)–mass spectrometry and GC-flame ionization detection. The major components in the essential oils were α-pinene, ( Z)-β-ocimene, ( E)-β-ocimene, β-caryophyllene, germacrene D, bicyclogermacrene, and spathulenol. The essential oils were screened for antimicrobial activity against Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans, as well as mosquito larvicidal activity against Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus. Actinodaphne pilosa leaf essential oils showed broad antimicrobial activity (minimum inhibitory concentration = 32, 64, 64, 16, and 16 μg/mL against E. faecalis, S. aureus, B. cereus, P. aeruginosa, and C. albicans, respectively) and excellent larvicidal activity (24-hour 50% lethal concentration = 19.0, 24.7, and 48.1 μg/mL against A. aegypti, A. albopictus, and C. quinquefasciatus, respectively).