Dose-response effects of the Savory (Satureja khuzistanica) essential oil and extract on rumen fermentation characteristics, microbial protein synthesis and methane production in vitro

The objective of the present study was to investigate dose-response effects of the essential oil (EO) and dry extract (EX) of Satureja khuzistanica (SK) on in vitro gas production kinetics, rumen fermentation, ruminal methanogenesis and microbial protein synthesis. So, EO and EX were tested at 0 (as control); 150 (low dose); 300, 450 (intermediate doses) and 600 mg/L (high dose). The gas produced over 24 h of incubation (GP24) decreased linearly with both EO and EX dosages (PIn vitro methane production was reduced by both EO (14–69%, depending on the included dose) and EX (7–58%). Microbial protein (MP) as well as the efficiency of microbial protein synthesis (EMPS) were improved by EO (18.8–49.8% and 20.4–61.5% for MP and EMPS, respectively) and to a lesser extent by EX (8.3–25.7% and 4.6–24.2% for MP and EMPS, respectively). Ammonia concentration was dropped in linear and quadratic manners with EO (Pin vivo experiments.