Synergistic Effect of Thymus vulgaris Essential Oil Oral Administration on Topically Treated Wound with Chitosan, Thyme Essential Oil and their Combination in Rats

Herbal medication from natural resources plays an important role as antioxidant, antibacterial agent and demonstrating an inimitable property in wound curative rather than synthesized-toxic materials. The present investigation was designed to evaluate the potential healing efficacy of thyme (Thymus vulgaris) essential oil (TEO) oral adminstartion on topically treated wound with chitosan, TEO and their combination in vitro. Practically, effect of TEO oral administration on topically treated wounded rats in comparing to topical application of betadine for two weeks was carried out. Remarkably, treated group with TEO topically and orally exhibited the highest wound contractions among all treated groups. In Parallel, histopathological alteration was improved in TEO topically and orally applications whereas no degeneration, necrosis & inflammatory cells infiltration in skeletal muscle of wound with rejuvenation of hair follicles were observed. Wound healing efficiency was established in vivo and recommended as natural agent for wounds through protection of injury site from infections and inhibition of inflammatory cells as well as increasing connective tissue formation in repaired tissues. These favored results confirm that treated group with TEO topically combined with TEO orally have outstanding wound healing activity and need exploitation as an alternative source of natural wound agents for medical applications.