ODREĐIVANJE HAMAZULENA U ETARSKOM ULJU KAMILICE PRIMENOM HROMATOGRAFSKOSPEKTROFOTOMETRIJSKOG METODA. (Croatian)
In: Bulletin for Hops, Sorghum & Medicinal Plants, Jg. 45 (2013-10-01), Heft 86, S. 24-32
academicJournal
Zugriff:
In this paper a method based on silica gel G thin layer chromatography and UV-spectrophotometry for chamazulene determination in chamomile inflorescences essential oil was developed. The essential oil was obtained by hydrodistillation of plant material while cyclohexane was used as the mobile phase for the separation of chamazulene. The use of 95 % (w/w) ethanol is suitable for the extraction of chamazulene from the silica gel, in which the chamazulene content can later be determined directly by measuring absorbance at 284 nm. This combined chromatographic-spectrophotometric method ensures high reproduction of produced results (KW = 2.36 %). For that reason, this method was used for chamazulene determination in essential oil of chamomile inflorescences, collected from different sites in Vojvodina. It was found that the chamazulene content in the examined samples of essential oil was between 3.32 and 8.76 %. [ABSTRACT FROM AUTHOR]
Razrađen je metod za određivanje hamazulena u etarskom ulju cvasti kamilice, koji se zasniva na primeni hromatografije na tankom sloju silikagela G i UV-spektrometrije. Etarsko ulje iz biljnog materijala se dobija postupkom hidrodestilacije, a za razdvajanje hamazulena korišćen je cikloheksan kao pokretna faza. Za ekstrakciju hamazulena sa silikagela pogodan je etanol koncentracije 95 % (m/m), u kome se sadržaj hamazulena određuje direktnim merenjem apsorbance na 284 nm. Razrađeni kombinovani hromatografsko-spektrometrijski metod obezbeđuje visok stepen reprodukcije rezultata (Kw=2,36 %). Primenom ovog metoda određen je sadržaj hamazulena u etarskom ulju cvasti, dobijenih od kamilice sakupljene sa različitih staništa u Vojvodini. Nađeno je da ispitivani uzorci etarskog ulja sadrže hamazulen od 3,32 do 8,76 %. [ABSTRACT FROM AUTHOR]
Copyright of Bulletin for Hops, Sorghum & Medicinal Plants is the property of Institute of Field & Vegetable Crops and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
Titel: |
ODREĐIVANJE HAMAZULENA U ETARSKOM ULJU KAMILICE PRIMENOM HROMATOGRAFSKOSPEKTROFOTOMETRIJSKOG METODA. (Croatian)
|
---|---|
Autor/in / Beteiligte Person: | Lepojević, Ivana ; Lepojević, Ž. ; Zeković, Z. ; Nađalin, Vesna ; Adamović, D. |
Zeitschrift: | Bulletin for Hops, Sorghum & Medicinal Plants, Jg. 45 (2013-10-01), Heft 86, S. 24-32 |
Veröffentlichung: | 2013 |
Medientyp: | academicJournal |
ISSN: | 0351-9430 (print) |
Sonstiges: |
|