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Transmembrane signaling by the phospholipase C-beta (PLC-beta) pathway is known to require at least three components: the receptor, the G protein, and the PLC. Recent studies have indicated that if the cytosol is allowed to leak out of HL60 cells, then G protein-stimulated PLC activity is greatly diminished, indicating an essential role for a cytosolic component(s). We now report the complete purification of one component based on its ability to reconstitute GTP gamma S-mediated PLC activity and identify it as the phosphatidylinositol transfer protein (PI-TP). Based on the in vitro effects of PI-TP, we surmise that it is involved in transporting PI from intracellular compartments for conversion to PI bisphosphate (PIP2) prior to hydrolysis by PLC-beta 2/PLC-beta 3, the endogenous PLC isoforms present in these cells.

Titel:	An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling.
Autor/in / Beteiligte Person:	Thomas, GM ; Cunningham, E ; Fensome, A ; Ball, A ; Totty, NF ; Truong, O ; Hsuan, JJ ; Cockcroft, S
Zeitschrift:	Cell, Jg. 74 (1993-09-10), Heft 5, S. 919-28
Veröffentlichung:	Cambridge, Ma : Cell Press ; <i>Original Publication</i>: Cambridge, MIT Press., 1993
Medientyp:	academicJournal
ISSN:	0092-8674 (print)
DOI:	10.1016/0092-8674(93)90471-2
Schlagwort:	Amino Acid Sequence Animals Carrier Proteins chemistry Carrier Proteins isolation & purification Cell Membrane metabolism Guanosine 5'-O-(3-Thiotriphosphate) pharmacology Humans Immunoblotting Kinetics Leukemia, Promyelocytic, Acute Molecular Sequence Data Phospholipid Transfer Proteins Rats Sequence Homology, Amino Acid Tumor Cells, Cultured Type C Phospholipases chemistry Type C Phospholipases isolation & purification Carrier Proteins metabolism GTP-Binding Proteins metabolism Membrane Proteins Phosphatidylinositols metabolism Saccharomyces cerevisiae Proteins Signal Transduction Type C Phospholipases metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Language: English [Cell] 1993 Sep 10; Vol. 74 (5), pp. 919-28. MeSH Terms: Membrane Proteins* ; Saccharomyces cerevisiae Proteins* ; Signal Transduction* ; Carrier Proteins / metabolism ; GTP-Binding Proteins / metabolism ; Phosphatidylinositols / metabolism ; Type C Phospholipases / metabolism ; Amino Acid Sequence ; Animals ; Carrier Proteins / chemistry ; Carrier Proteins / isolation & purification ; Cell Membrane / metabolism ; Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology ; Humans ; Immunoblotting ; Kinetics ; Leukemia, Promyelocytic, Acute ; Molecular Sequence Data ; Phospholipid Transfer Proteins ; Rats ; Sequence Homology, Amino Acid ; Tumor Cells, Cultured ; Type C Phospholipases / chemistry ; Type C Phospholipases / isolation & purification Grant Information: United Kingdom Wellcome Trust Substance Nomenclature: 0 (Carrier Proteins) ; 0 (Membrane Proteins) ; 0 (Phosphatidylinositols) ; 0 (Phospholipid Transfer Proteins) ; 0 (SEC24 protein, S cerevisiae) ; 0 (Saccharomyces cerevisiae Proteins) ; 37589-80-3 (Guanosine 5'-O-(3-Thiotriphosphate)) ; EC 3.1.4.- (Type C Phospholipases) ; EC 3.6.1.- (GTP-Binding Proteins) Entry Date(s): Date Created: 19930910 Date Completed: 19931015 Latest Revision: 20190705 Update Code: 20240513

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An essential role for phosphatidylinositol transfer protein in phospholipase C-mediated inositol lipid signaling.