Evidence that asgB encodes a DNA-binding protein essential for growth and development of Myxococcus xanthus.
In: Journal of bacteriology, Jg. 176 (1994-04-01), Heft 7, S. 2013-20
academicJournal
Zugriff:
The asg mutants of Myxococcus xanthus are defective in production of extracellular A-signal, which serves as a cell density signal for fruiting-body development. The DNA sequence of asgB, one of the three asg genes, was determined. The deduced amino acid sequence of AsgB contains a DNA-binding helix-turn-helix motif near the C terminus. This putative helix-turn-helix is highly similar to the helix-turn-helix in region 4.2 of major sigma factors, which is the region that recognizes and interacts with -35 sequences of promoters. We propose that AsgB is a transcription factor that binds to DNA sequences similar to the -35 hexamer, TTGACA. Analyses of asgB RNA levels and expression of an asgB-lacZ translational fusion indicate that expression of asgB remains fairly constant during the transition from growth into early development. The mutation within the asgB480 allele was identified as an A-to-G transition that results in a threonine-to-alanine substitution in the predicted protein product. Attempts to replace the wild-type copy of asgB with a null allele failed, indicating that asgB may be essential for growth.
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Evidence that asgB encodes a DNA-binding protein essential for growth and development of Myxococcus xanthus.
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Autor/in / Beteiligte Person: | Plamann, L ; Davis, JM ; Cantwell, B ; Mayor, J |
Zeitschrift: | Journal of bacteriology, Jg. 176 (1994-04-01), Heft 7, S. 2013-20 |
Veröffentlichung: | Washington, DC : American Society for Microbiology, 1994 |
Medientyp: | academicJournal |
ISSN: | 0021-9193 (print) |
DOI: | 10.1128/jb.176.7.2013-2020.1994 |
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