Elevated expression of the junB proto-oncogene is essential for v-fos induced transformation of Rat-1 cells.
In: Oncogene, Jg. 9 (1994-10-01), Heft 10, S. 2969-76
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Zugriff:
We previously described the isolation of non-tumorigenic revertants from mutagenized populations of v-fos-transformed Rat-1 cells (Zarbl et al., 1987). In the present study we examined the possibility that the revertant phenotype resulted from mutations that altered the expression or activities of the c-jun or junB proto-oncogenes. The results demonstrated that levels of the c-jun mRNA and protein were unchanged in the revertants when compared to the transformed parental cells, and ectopic overexpression of c-jun failed to retransform the revertants. Although one mutant allele was detected in revertant EMS-1-19, overexpression of this mutant allele failed to inhibit v-fos induced cell transformation. Together these results indicated that the revertant phenotype did not result from altered expression or mutations in the c-jun gene. In contrast to the results obtained with c-jun, the levels of junB mRNA and protein were found to be reduced two- or threefold in revertant EMS-1-19. Ectopic overexpression of junB induced transformation of revertant EMS-1-19, but failed to transform Rat-1 cells. Moreover, about 10% of v-fos transformed cells transfected with vectors that express antisense junB mRNA acquired a non-transformed phenotype. Together these results indicate that expression of junB above a threshold level is essential for v-fos-induced transformation of Rat-1 fibroblasts.
Titel: |
Elevated expression of the junB proto-oncogene is essential for v-fos induced transformation of Rat-1 cells.
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Autor/in / Beteiligte Person: | Van Amsterdam, JR ; Wang, Y ; Sullivan, RC ; Zarbl, H |
Zeitschrift: | Oncogene, Jg. 9 (1994-10-01), Heft 10, S. 2969-76 |
Veröffentlichung: | <2002->: Basingstoke : Nature Publishing Group ; <i>Original Publication</i>: Basingstoke, Hampshire, UK : Scientific & Medical Division, MacMillan Press, c1987-, 1994 |
Medientyp: | academicJournal |
ISSN: | 0950-9232 (print) |
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