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The postulated central pseudoknot formed by regions 9-13/21-25 and 17-19/916-918 of 16S rRNA of Escherichia coli is phylogenetically conserved in prokaryotic as well eukaryotic species. This pseudoknot is located at the center of the secondary structure of the 16S rRNA and connects the three major domains of this molecule. We have introduced mutations into this pseudoknot by changing the base-paired residues C18 and G917, and the effect of such mutations on the ribosomal activity was studied in vivo, using a 'specialized' ribosome system. As compared with ribosomes having the wild-type pseudoknot, the translational activity of ribosomes containing an A, G or U residue at position 18 was dramatically reduced, while the activity of mutant ribosomes having complementary bases at positions 18 and 917 was at the wild-type level. The reduced translational activity of those mutants that are incapable of forming a pseudoknot was caused by their inability to form 70S ribosomal complexes. These results demonstrate that the potential formation of a central pseudoknot in 16S rRNA with any base-paired residues at positions 18 and 917 is essential to complete the initiation process.

Titel:	Formation of the central pseudoknot in 16S rRNA is essential for initiation of translation.
Autor/in / Beteiligte Person:	Brink, MF ; Verbeet, MP ; de Boer HA
Zeitschrift:	The EMBO journal, Jg. 12 (1993-10-01), Heft 10, S. 3987-96
Veröffentlichung:	2024- : [London] : Nature Publishing Group ; <i>Original Publication</i>: Eynsham, Oxford, England : Published for the European Molecular Biology Organization by IRL Press, [c1982-, 1993
Medientyp:	academicJournal
ISSN:	0261-4189 (print)
DOI:	10.1002/j.1460-2075.1993.tb06076.x
Schlagwort:	Base Composition Base Sequence DNA, Bacterial Molecular Sequence Data Mutagenesis, Site-Directed Phylogeny RNA Precursors metabolism RNA Processing, Post-Transcriptional RNA, Bacterial RNA, Ribosomal, 16S chemistry Restriction Mapping Ribosomes metabolism Escherichia coli genetics Nucleic Acid Conformation Protein Biosynthesis RNA, Ribosomal, 16S genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [EMBO J] 1993 Oct; Vol. 12 (10), pp. 3987-96. MeSH Terms: Nucleic Acid Conformation* ; Protein Biosynthesis* ; Escherichia coli / genetics ; RNA, Ribosomal, 16S / genetics ; Base Composition ; Base Sequence ; DNA, Bacterial ; Molecular Sequence Data ; Mutagenesis, Site-Directed ; Phylogeny ; RNA Precursors / metabolism ; RNA Processing, Post-Transcriptional ; RNA, Bacterial ; RNA, Ribosomal, 16S / chemistry ; Restriction Mapping ; Ribosomes / metabolism References: EMBO J. 1986 May;5(5):1111-3. (PMID: 3720727) ; J Mol Biol. 1986 Feb 5;187(3):399-416. (PMID: 2422386) ; DNA. 1987 Apr;6(2):173-8. (PMID: 3472867) ; Proc Natl Acad Sci U S A. 1987 Jul;84(14):4757-61. (PMID: 2440027) ; Proc Natl Acad Sci U S A. 1987 Jul;84(14):4762-6. (PMID: 2440028) ; Nature. 1988 Jan 21;331(6153):283-6. (PMID: 3336440) ; Methods Enzymol. 1987;153:432-52. (PMID: 2828845) ; J Mol Biol. 1988 Jan 5;199(1):115-36. (PMID: 2451022) ; Proc Natl Acad Sci U S A. 1988 Oct;85(19):7144-8. (PMID: 3050989) ; J Mol Biol. 1988 Nov 20;204(2):447-81. (PMID: 2464693) ; Methods Enzymol. 1988;164:673-90. (PMID: 3071688) ; Proc Natl Acad Sci U S A. 1989 May;86(9):3119-22. (PMID: 2654936) ; Cell. 1989 Jul 14;58(1):9-12. (PMID: 2473840) ; Nature. 1989 Nov 9;342(6246):142-8. (PMID: 2682263) ; J Mol Biol. 1989 Oct 20;209(4):645-53. (PMID: 2531227) ; Proc Natl Acad Sci U S A. 1990 Feb;87(3):1042-6. (PMID: 2405392) ; Nucleic Acids Res. 1990 Apr 25;18 Suppl:2237-317. (PMID: 1692117) ; Trends Biochem Sci. 1990 Apr;15(4):143-7. (PMID: 1692647) ; Nucleic Acids Res. 1990 Jul 25;18(14):4083-8. (PMID: 2198532) ; FEBS Lett. 1991 Feb 25;279(2):171-4. (PMID: 2001727) ; EMBO J. 1991 Aug;10(8):2203-14. (PMID: 1712293) ; J Mol Biol. 1991 Oct 5;221(3):889-907. (PMID: 1942035) ; Biochemistry. 1992 Dec 1;31(47):11665-76. (PMID: 1280160) ; Genes Dev. 1993 Apr;7(4):660-70. (PMID: 7681419) ; Nature. 1974 Feb 22;247(5442):552-4. (PMID: 4594437) ; Biochemistry. 1978 Feb 21;17(4):587-93. (PMID: 341969) ; J Mol Biol. 1982 Jul 25;159(1):151-66. (PMID: 6182299) ; Nucleic Acids Res. 1984 Jun 11;12(11):4653-63. (PMID: 6330677) ; Nucleic Acids Res. 1985 Mar 11;13(5):1717-31. (PMID: 4000943) ; J Mol Biol. 1985 Jul 5;184(1):53-66. (PMID: 2411936) ; Proc Natl Acad Sci U S A. 1986 Dec;83(24):9373-7. (PMID: 2432595) Substance Nomenclature: 0 (DNA, Bacterial) ; 0 (RNA Precursors) ; 0 (RNA, Bacterial) ; 0 (RNA, Ribosomal, 16S) Entry Date(s): Date Created: 19931001 Date Completed: 19931027 Latest Revision: 20220511 Update Code: 20240513 PubMed Central ID: PMC413681

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Formation of the central pseudoknot in 16S rRNA is essential for initiation of translation.