Einzeltreffer — DigiBib

To investigate cell cycle regulation at the S or G2 phase in Saccharomyces cerevisiae, we have isolated mutants displaying supersensitivity to hydroxyurea (HU), a chemical that inhibits DNA replication. Such mutants, which we have named hydroxyurea sensitive (hys), defined four linkage groups and we characterized the hys2 mutation in this study. The hys2-1 mutant displays temperature sensitive growth and a constellation of phenotypes indicating defective DNA metabolism. At the restrictive temperature, hys2-1 cells arrest as large budded cells with a single nucleus at the neck of the bud and a short spindle. The hys2-1 mutant exhibits increased rates of chromosome loss and recombination. Additionally, hys2-1 appears to accumulate incompletely replicated DNA that can be detected by a pulse field electrophoresis assay. Finally, deletion of RAD9 in a hys2-1 strain decreases the percentage of arrested cells, suggesting that an intact RAD9-checkpoint is required for the cell cycle arrest in hys2-1 cells. HYS2 encodes a 55 kDa protein that is essential for viability at all temperatures. Taken together, these data suggest that Hys2 plays a role in DNA replication.

Titel:	HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae.
Autor/in / Beteiligte Person:	Sugimoto, K ; Sakamoto, Y ; Takahashi, O ; Matsumoto, K
Zeitschrift:	Nucleic acids research, Jg. 23 (1995-09-11), Heft 17, S. 3493-500
Veröffentlichung:	1992- : Oxford : Oxford University Press ; <i>Original Publication</i>: London, Information Retrieval ltd., 1995
Medientyp:	academicJournal
ISSN:	0305-1048 (print)
DOI:	10.1093/nar/23.17.3493
Schlagwort:	Base Sequence Cell Cycle Cell Cycle Proteins chemistry Cloning, Molecular DNA Polymerase III DNA Primers chemistry DNA, Fungal biosynthesis Enzyme Inhibitors pharmacology Fungal Proteins chemistry Gene Expression Regulation, Fungal Hydroxyurea pharmacology Molecular Sequence Data RNA, Messenger genetics Cell Cycle Proteins genetics DNA Replication DNA-Directed DNA Polymerase Fungal Proteins genetics Genes, Fungal Saccharomyces cerevisiae genetics Saccharomyces cerevisiae Proteins
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Nucleic Acids Res] 1995 Sep 11; Vol. 23 (17), pp. 3493-500. MeSH Terms: DNA Replication* ; DNA-Directed DNA Polymerase* ; Genes, Fungal* ; Saccharomyces cerevisiae Proteins* ; Cell Cycle Proteins / genetics ; Fungal Proteins / genetics ; Saccharomyces cerevisiae / *genetics ; Base Sequence ; Cell Cycle ; Cell Cycle Proteins / chemistry ; Cloning, Molecular ; DNA Polymerase III ; DNA Primers / chemistry ; DNA, Fungal / biosynthesis ; Enzyme Inhibitors / pharmacology ; Fungal Proteins / chemistry ; Gene Expression Regulation, Fungal ; Hydroxyurea / pharmacology ; Molecular Sequence Data ; RNA, Messenger / genetics References: Science. 1989 Nov 3;246(4930):629-34. (PMID: 2683079) ; EMBO J. 1989 Jun;8(6):1849-54. (PMID: 2670563) ; Cell. 1990 Jul 27;62(2):225-37. (PMID: 2142620) ; Genes Dev. 1990 May;4(5):740-51. (PMID: 2199320) ; Gene. 1990 May 31;90(1):99-104. (PMID: 2199334) ; Mol Cell Biol. 1991 Feb;11(2):987-1001. (PMID: 1899289) ; Cell. 1991 Mar 22;64(6):1111-22. (PMID: 1706223) ; Cell. 1991 May 31;65(5):875-83. (PMID: 2040016) ; Cell. 1991 Jun 14;65(6):921-3. (PMID: 2044152) ; Genes Dev. 1991 Jun;5(6):958-69. (PMID: 2044962) ; Nature. 1992 Jan 23;355(6358):365-8. (PMID: 1731250) ; Nature. 1992 Jan 23;355(6358):368-71. (PMID: 1731251) ; Nucleic Acids Res. 1992 May 25;20(10):2403-10. (PMID: 1598198) ; Nature. 1992 Oct 15;359(6396):599-604. (PMID: 1406993) ; Genes Dev. 1992 Nov;6(11):2035-46. (PMID: 1427071) ; Genetics. 1993 May;134(1):63-80. (PMID: 8514150) ; Mol Cell Biol. 1994 Jan;14(1):255-67. (PMID: 8264593) ; EMBO J. 1994 Jan 1;13(1):61-70. (PMID: 8306972) ; Bioessays. 1993 Dec;15(12):775-82. (PMID: 8141795) ; Mol Cell Biol. 1994 May;14(5):3158-65. (PMID: 8164671) ; Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9047-51. (PMID: 8090767) ; Genes Dev. 1994 Mar 15;8(6):652-65. (PMID: 7926756) ; Genes Dev. 1994 Oct 15;8(20):2416-28. (PMID: 7958906) ; J Mol Biol. 1976 Jul 15;104(4):803-17. (PMID: 785015) ; Nature. 1978 Aug 31;274(5674):891-3. (PMID: 355897) ; J Bacteriol. 1983 Jan;153(1):163-8. (PMID: 6336730) ; Methods Enzymol. 1983;101:202-11. (PMID: 6310324) ; Genetics. 1985 Jul;110(3):381-95. (PMID: 3894160) ; Nucleic Acids Res. 1985 Dec 9;13(23):8323-37. (PMID: 3909103) ; Science. 1988 Jul 15;241(4863):317-22. (PMID: 3291120) ; Mol Gen Genet. 1988 Jun;212(3):459-65. (PMID: 3047550) ; J Cell Sci. 1988 Mar;89 ( Pt 3):343-57. (PMID: 3198697) ; Cell. 1989 Feb 24;56(4):599-605. (PMID: 2645055) ; Proc Natl Acad Sci U S A. 1989 Apr;86(8):2784-8. (PMID: 2649894) ; Mol Cell Biol. 1989 Feb;9(2):365-76. (PMID: 2651896) ; Gene. 1988 Dec 30;74(2):527-34. (PMID: 3073106) ; Mol Cell Biol. 1989 May;9(5):1882-96. (PMID: 2664461) ; Proc Natl Acad Sci U S A. 1989 Aug;86(16):6255-9. (PMID: 2569741) ; Cell. 1990 Feb 23;60(4):665-73. (PMID: 2406029) Molecular Sequence: GENBANK D50324 Substance Nomenclature: 0 (Cell Cycle Proteins) ; 0 (DNA Primers) ; 0 (DNA, Fungal) ; 0 (Enzyme Inhibitors) ; 0 (Fungal Proteins) ; 0 (RNA, Messenger) ; 0 (Saccharomyces cerevisiae Proteins) ; EC 2.7.7.7 (DNA Polymerase III) ; EC 2.7.7.7 (DNA-Directed DNA Polymerase) ; EC 2.7.7.7 (POL31 protein, S cerevisiae) ; X6Q56QN5QC (Hydroxyurea) Entry Date(s): Date Created: 19950911 Date Completed: 19951106 Latest Revision: 20201209 Update Code: 20240513 PubMed Central ID: PMC307229

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

HYS2, an essential gene required for DNA replication in Saccharomyces cerevisiae.