Einzeltreffer — DigiBib

Cohesin is a multi-subunit protein that plays a pivotal role in holding sister chromatids together during cell division. Sister chromatid cohesion 3 (SCC3), constituents of cohesin complex, is highly conserved from yeast to mammals. Since the deletion of individual cohesin subunit always causes lethality, it is difficult to dissect its biological function in both mitosis and meiosis. Here, we obtained scc3 weak mutants using CRISPR-Cas9 system to explore its function during rice mitosis and meiosis. The scc3 weak mutants displayed obvious vegetative defects and complete sterility, underscoring the essential roles of SCC3 in both mitosis and meiosis. SCC3 is localized on chromatin from interphase to prometaphase in mitosis. However, in meiosis, SCC3 acts as an axial element during early prophase I and subsequently situates onto centromeric regions following the disassembly of the synaptonemal complex. The loading of SCC3 onto meiotic chromosomes depends on REC8. scc3 shows severe defects in homologous pairing and synapsis. Consequently, SCC3 functions as an axial element that is essential for maintaining homologous chromosome pairing and synapsis during meiosis.

Competing Interests: YZ, LR, TZ, HY, YM, HL, LC, BW, YS, YL, DT, ZC No competing interests declared

Titel:	SCC3 is an axial element essential for homologous chromosome pairing and synapsis.
Autor/in / Beteiligte Person:	Zhao, Y ; Ren, L ; Zhao, T ; You, H ; Miao, Y ; Liu, H ; Cao, L ; Wang, B ; Shen, Y ; Li, Y ; Tang, D ; Cheng, Z
Zeitschrift:	ELife, Jg. 13 (2024-06-12)
Veröffentlichung:	Cambridge, UK : eLife Sciences Publications, Ltd., 2012-, 2024
Medientyp:	academicJournal
ISSN:	2050-084X (electronic)
DOI:	10.7554/eLife.94180
Schlagwort:	Cohesins Mitosis Synaptonemal Complex metabolism Synaptonemal Complex genetics CRISPR-Cas Systems Chromosome Pairing Meiosis genetics Cell Cycle Proteins metabolism Cell Cycle Proteins genetics Oryza genetics Chromosomal Proteins, Non-Histone metabolism Chromosomal Proteins, Non-Histone genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Elife] 2024 Jun 12; Vol. 13. <i>Date of Electronic Publication: </i>2024 Jun 12. MeSH Terms: Chromosome Pairing* ; Meiosis* / genetics ; Cell Cycle Proteins* / metabolism ; Cell Cycle Proteins* / genetics ; Oryza* / genetics ; Chromosomal Proteins, Non-Histone* / metabolism ; Chromosomal Proteins, Non-Histone* / genetics ; Cohesins ; Mitosis ; Synaptonemal Complex / metabolism ; Synaptonemal Complex / genetics ; CRISPR-Cas Systems References: Genes (Basel). 2022 Jan 22;13(2):. (PMID: 35205243) ; Methods Mol Biol. 2013;990:125-34. (PMID: 23559209) ; Nature. 2010 Sep 23;467(7314):430-5. (PMID: 20720539) ; Plant Physiol. 2021 Nov 3;187(3):1605-1618. (PMID: 34618076) ; J Biol Chem. 2004 Feb 20;279(8):6553-9. (PMID: 14660624) ; J Cell Biol. 2009 Oct 19;187(2):165-73. (PMID: 19822671) ; PLoS Genet. 2020 Sep 15;16(9):e1009048. (PMID: 32931493) ; Mol Cell. 2013 Aug 22;51(4):440-53. (PMID: 23973374) ; Plant Cell. 2012 Dec;24(12):4961-73. (PMID: 23243128) ; J Cell Sci. 2005 Oct 15;118(Pt 20):4621-32. (PMID: 16176934) ; Cell. 2015 Dec 17;163(7):1628-40. (PMID: 26687354) ; EMBO J. 2012 May 2;31(9):2090-102. (PMID: 22415368) ; Trends Cell Biol. 2016 Sep;26(9):680-693. (PMID: 27134029) ; Front Plant Sci. 2017 May 23;8:846. (PMID: 28588601) ; Nucleic Acids Res. 2015 Mar 31;43(6):3056-67. (PMID: 25735743) ; PLoS Genet. 2015 Mar 06;11(3):e1005036. (PMID: 25748820) ; Plant Cell. 2018 Dec;30(12):3024-3037. (PMID: 30538156) ; Genes Cells. 2019 Jan;24(1):6-30. (PMID: 30479058) ; Mol Cell. 2020 Sep 17;79(6):917-933.e9. (PMID: 32755595) ; Annu Rev Plant Biol. 2015;66:297-327. (PMID: 25494464) ; Genome Res. 2020 Apr;30(4):515-527. (PMID: 32253279) ; Proc Natl Acad Sci U S A. 2018 Oct 16;115(42):10810-10815. (PMID: 30275327) ; Cell. 1999 Jul 9;98(1):91-103. (PMID: 10412984) ; Annu Rev Genet. 2009;43:525-58. (PMID: 19886810) ; Plant Cell. 2020 Apr;32(4):1218-1239. (PMID: 32024691) ; EMBO J. 2021 Apr 1;40(7):e106797. (PMID: 33644892) ; EMBO J. 2014 Jun 2;33(11):1243-55. (PMID: 24797475) ; Plant Physiol. 2020 Aug;183(4):1517-1530. (PMID: 32554471) ; Cell. 1997 Oct 3;91(1):35-45. (PMID: 9335333) ; Plant J. 2020 Aug;103(4):1446-1459. (PMID: 32391618) ; Plant Physiol. 2016 May;171(1):230-41. (PMID: 26960731) ; Cell. 2000 Dec 22;103(7):1155-68. (PMID: 11163190) ; Dev Cell. 2021 Nov 22;56(22):3100-3114.e4. (PMID: 34758289) ; PLoS Genet. 2016 Oct 28;12(10):e1006389. (PMID: 27792785) ; Elife. 2018 Aug 15;7:. (PMID: 30109982) ; Genes Dev. 2007 Sep 1;21(17):2220-33. (PMID: 17785529) ; J Cell Sci. 2007 Feb 1;120(Pt 3):367-9. (PMID: 17251376) ; FEBS Lett. 2014 Oct 16;588(20):3692-702. (PMID: 25171859) ; New Phytol. 2018 Apr;218(2):789-803. (PMID: 29479720) ; PLoS Genet. 2014 Jul 03;10(7):e1004413. (PMID: 24992337) ; PLoS Genet. 2012;8(8):e1002856. (PMID: 22912589) ; Curr Genet. 2021 Jun;67(3):447-459. (PMID: 33404730) ; J Cell Sci. 2003 Jul 15;116(Pt 14):2999-3007. (PMID: 12783989) ; PLoS Biol. 2010 Oct 19;8(10):e1000520. (PMID: 20976044) ; Essays Biochem. 2019 Apr 23;63(1):167-176. (PMID: 31015387) ; Genes Dev. 2010 Feb 1;24(3):219-28. (PMID: 20123904) ; Trends Genet. 2005 Jul;21(7):405-12. (PMID: 15946764) ; EMBO Rep. 2016 Jun;17(6):901-13. (PMID: 27170622) ; PLoS Genet. 2020 Jun 9;16(6):e1008849. (PMID: 32516352) ; Nature. 1999 Jul 29;400(6743):461-4. (PMID: 10440376) ; Plant Physiol. 2011 Jul;156(3):1386-96. (PMID: 21606318) ; J Cell Biol. 2012 Jun 25;197(7):877-85. (PMID: 22711701) ; Plant J. 2011 Aug;67(4):583-94. (PMID: 21615569) ; Plant Cell. 2012 May;24(5):2058-70. (PMID: 22589466) ; Elife. 2024 Jun 12;13:. (PMID: 38864853) ; Plant J. 2021 Nov;108(3):661-671. (PMID: 34397127) ; Cell Rep. 2021 Nov 2;37(5):109941. (PMID: 34731625) Grant Information: 2023YFA0913500 National Key Research and Development Program of China; U2102219 National Natural Science Foundation of China; 31930018 National Natural Science Foundation of China; 32201779 National Natural Science Foundation of China Contributed Indexing: Keywords: cell biology; genetics; genomics; meiocyte; meiosis; rice Molecular Sequence: Dryad 10.5061/dryad.kh18932fz Substance Nomenclature: 0 (Cell Cycle Proteins) ; 0 (Chromosomal Proteins, Non-Histone) ; 0 (Cohesins) Entry Date(s): Date Created: 20240612 Date Completed: 20240612 Latest Revision: 20240620 Update Code: 20240621 PubMed Central ID: PMC11168746

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SCC3 is an axial element essential for homologous chromosome pairing and synapsis.