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Plasmodium sporozoites are the infective forms of the malaria parasite in the mosquito and vertebrate host. Gliding motility allows sporozoites to migrate and invade mosquito salivary glands and mammalian hosts. Motility and invasion are powered by an actin-myosin motor complex linked to the glideosome, which contains glideosome-associated proteins (GAPs), MyoA and the myosin A tail-interacting protein (MTIP). However, the role of several proteins involved in gliding motility remains unknown. We identified that the S14 gene is upregulated in sporozoite from transcriptome data of Plasmodium yoelii and further confirmed its transcription in P. berghei sporozoites using real-time PCR. C-terminal 3×HA-mCherry tagging revealed that S14 is expressed and localized on the inner membrane complex of the sporozoites. We disrupted S14 in P. berghei and demonstrated that it is essential for sporozoite gliding motility, and salivary gland and hepatocyte invasion. The gliding and invasion-deficient S14 knockout sporozoites showed normal expression and organization of inner membrane complex and surface proteins. Taken together, our data show that S14 plays a role in the function of the glideosome and is essential for malaria transmission.

Competing Interests: Competing interests The authors declare no competing or financial interests.

Titel:	The novel Plasmodium berghei protein S14 is essential for sporozoite gliding motility and infectivity.
Autor/in / Beteiligte Person:	Ghosh, A ; Varshney, A ; Narwal, SK ; Nirdosh ; Gupta, R ; Mishra, S
Zeitschrift:	Journal of cell science, Jg. 137 (2024-06-01), Heft 11
Veröffentlichung:	Cambridge : Company of Biologists ; <i>Original Publication</i>: London., 2024
Medientyp:	academicJournal
ISSN:	1477-9137 (electronic)
DOI:	10.1242/jcs.261857
Schlagwort:	Animals Mice Salivary Glands parasitology Salivary Glands metabolism Anopheles parasitology Sporozoites metabolism Plasmodium berghei metabolism Plasmodium berghei genetics Protozoan Proteins metabolism Protozoan Proteins genetics Malaria parasitology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Cell Sci] 2024 Jun 01; Vol. 137 (11). <i>Date of Electronic Publication: </i>2024 Jun 07. MeSH Terms: Sporozoites* / metabolism ; Plasmodium berghei* / metabolism ; Plasmodium berghei* / genetics ; Protozoan Proteins* / metabolism ; Protozoan Proteins* / genetics ; Malaria* / parasitology ; Animals ; Mice ; Salivary Glands / parasitology ; Salivary Glands / metabolism ; Anopheles / parasitology Grant Information: BT/RLF/Re-entry/20/2012 Department of Biotechnology, Ministry of Science and Technology, India; CRG/2022/003848 Science and Engineering Research Baord; 6/9-7(306)/2022/ECD-II Indian Council of Medical Research; CRG/2022/003848 Science and Engineering Research Board Contributed Indexing: Keywords: Plasmodium; Glideosome; Gliding motility; Inner membrane complex; Invasion; S14; Sporozoite Substance Nomenclature: 0 (Protozoan Proteins) Entry Date(s): Date Created: 20240604 Date Completed: 20240607 Latest Revision: 20240607 Update Code: 20240607

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The novel Plasmodium berghei protein S14 is essential for sporozoite gliding motility and infectivity.