Chemical profile, antibacterial and antioxidant properties of Rubia tinctorum L. essential oils.

Plants have gained great importance. Secondary metabolites contribute to the drug discovery and development by their bioactive properties. Rubia tinctorum L. essential oil (EO) was obtained and analysed. Antioxidant and antibacterial activities were evaluated. The plant's EOs were obtained through steam distillation, and the compounds were identified using gas chromatography-mass spectrometry (GC-MS) analysis. DPPH free radical scavenging and ferric-reducing antioxidant power (FRAP) were employed to assess antioxidant activity. Total antioxidant capacity (TAC) was also presented. The disc diffusion method was employed for testing antibacterial activity. Cyclohexanone was identified as the predominant component in the EO, constituting 88.74% of the total composition. The EO did not show significant antioxidant capacity, while it demonstrated antimicrobial effect against Bacillus cereus ATCC 6633 (>13 mm of inhibition; 500 mg/mL) and Shigella ATCC 12022 (≥12 mm of inhibition; 500 mg/mL). R. tinctorum L. is new source of cyclohexanone.