Einzeltreffer — DigiBib

Telomeres, which are chromosomal end structures, play a crucial role in maintaining genome stability and integrity in eukaryotes. In the baker's yeast Saccharomyces cerevisiae , the X- and Y'-elements are subtelomeric repetitive sequences found in all 32 and 17 telomeres, respectively. While the Y'-elements serve as a backup for telomere functions in cells lacking telomerase, the function of the X-elements remains unclear. This study utilized the S. cerevisiae strain SY12, which has three chromosomes and six telomeres, to investigate the role of X-elements (as well as Y'-elements) in telomere maintenance. Deletion of Y'-elements (SY12 YΔ ), X-elements (SY12 XYΔ+Y ), or both X- and Y'-elements (SY12 XYΔ ) did not impact the length of the terminal TG 1-3 tracks or telomere silencing. However, inactivation of telomerase in SY12 YΔ , SY12 XYΔ+Y , and SY12 XYΔ cells resulted in cellular senescence and the generation of survivors. These survivors either maintained their telomeres through homologous recombination-dependent TG 1-3 track elongation or underwent microhomology-mediated intra-chromosomal end-to-end joining. Our findings indicate the non-essential role of subtelomeric X- and Y'-elements in telomere regulation in both telomerase-proficient and telomerase-null cells and suggest that these elements may represent remnants of S. cerevisiae genome evolution. Furthermore, strains with fewer or no subtelomeric elements exhibit more concise telomere structures and offer potential models for future studies in telomere biology.

Competing Interests: CH, XZ, MH, YS, ZQ, ZW, JZ No competing interests declared

Titel:	Elimination of subtelomeric repeat sequences exerts little effect on telomere essential functions in Saccharomyces cerevisiae .
Autor/in / Beteiligte Person:	Hu, C ; Zhu, XT ; He, MH ; Shao, Y ; Qin, Z ; Wu, ZJ ; Zhou, JQ
Zeitschrift:	ELife, Jg. 12 (2024-04-24)
Veröffentlichung:	Cambridge, UK : eLife Sciences Publications, Ltd., 2012-, 2024
Medientyp:	academicJournal
ISSN:	2050-084X (electronic)
DOI:	10.7554/eLife.91223
Schlagwort:	Telomere Homeostasis Saccharomyces cerevisiae Proteins genetics Saccharomyces cerevisiae Proteins metabolism Sequence Deletion Saccharomyces cerevisiae genetics Telomere metabolism Telomere genetics Repetitive Sequences, Nucleic Acid genetics Telomerase genetics Telomerase metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Elife] 2024 Apr 24; Vol. 12. <i>Date of Electronic Publication: </i>2024 Apr 24. MeSH Terms: Saccharomyces cerevisiae* / genetics ; Telomere* / metabolism ; Telomere* / genetics ; Repetitive Sequences, Nucleic Acid* / genetics ; Telomerase* / genetics ; Telomerase* / metabolism ; Telomere Homeostasis ; Saccharomyces cerevisiae Proteins / genetics ; Saccharomyces cerevisiae Proteins / metabolism ; Sequence Deletion References: Mol Cell Biol. 1999 Dec;19(12):8083-93. (PMID: 10567534) ; Gravit Space Biol Bull. 2005 Jun;18(2):3-9. (PMID: 16038088) ; Genetics. 1999 May;152(1):143-52. (PMID: 10224249) ; Cell. 1989 May 19;57(4):633-43. (PMID: 2655926) ; Genetics. 2004 Apr;166(4):1701-13. (PMID: 15126391) ; PLoS Genet. 2015 Mar 30;11(3):e1005071. (PMID: 25822194) ; J Biol Chem. 1998 Dec 11;273(50):33360-6. (PMID: 9837911) ; Cell. 2023 Nov 22;186(24):5220-5236.e16. (PMID: 37944511) ; Annu Rev Genet. 2008;42:301-34. (PMID: 18680434) ; Nat Rev Genet. 2002 Nov;3(11):838-49. (PMID: 12415314) ; Genome Biol. 2002 Sep 27;3(10):REVIEWS0007. (PMID: 12372147) ; Mol Cell Biol. 1989 Dec;9(12):5754-7. (PMID: 2685577) ; PLoS Genet. 2014 Nov 06;10(11):e1004736. (PMID: 25375789) ; Science. 2017 Mar 10;355(6329):1040-1044. (PMID: 28280199) ; Elife. 2020 Aug 05;9:. (PMID: 32755541) ; Nat Rev Mol Cell Biol. 2004 Apr;5(4):323-9. (PMID: 15071557) ; Nucleic Acids Res. 2017 Oct 13;45(18):10333-10349. (PMID: 28981863) ; Genetics. 2012 Aug;191(4):1073-105. (PMID: 22879408) ; Cell. 1993 Apr 23;73(2):347-60. (PMID: 8477448) ; Nat Protoc. 2019 Aug;14(8):2521-2545. (PMID: 31300803) ; Mol Cell Biol. 2004 Nov;24(22):9887-98. (PMID: 15509791) ; Cell. 2016 May 19;165(5):1171-1181. (PMID: 27156450) ; Genetics. 1994 Mar;136(3):789-802. (PMID: 8005434) ; EMBO J. 2001 Feb 15;20(4):905-13. (PMID: 11179234) ; Mol Cell. 2000 Oct;6(4):947-52. (PMID: 11090632) ; Nat Struct Mol Biol. 2010 Dec;17(12):1478-85. (PMID: 21102445) ; Science. 1994 Oct 21;266(5184):404-9. (PMID: 7545955) ; Cell. 1993 Dec 17;75(6):1083-93. (PMID: 8261510) ; EMBO J. 1999 May 4;18(9):2522-37. (PMID: 10228166) ; Mol Cell. 2008 Aug 22;31(4):463-473. (PMID: 18722173) ; Genetics. 1996 Dec;144(4):1399-412. (PMID: 8978029) ; Genes Dev. 2004 Nov 1;18(21):2663-75. (PMID: 15489288) ; Nat Cell Biol. 2006 Jul;8(7):741-7. (PMID: 16767083) ; Yeast. 1995 Dec;11(16):1553-73. (PMID: 8720065) ; Nucleic Acids Res. 2019 Jun 20;47(11):5684-5697. (PMID: 30937455) ; Genetics. 2001 May;158(1):167-76. (PMID: 11333227) ; Nucleic Acids Res. 2017 Apr 7;45(6):3308-3322. (PMID: 28180293) ; Genetics. 1992 Jul;131(3):559-74. (PMID: 1628806) ; Cell. 1991 Sep 20;66(6):1279-87. (PMID: 1913809) ; Biochimie. 2008 Jan;90(1):93-107. (PMID: 17868970) ; Proc Natl Acad Sci U S A. 1981 Oct;78(10):6354-8. (PMID: 6273866) ; Nature. 2018 Aug;560(7718):331-335. (PMID: 30069045) ; J Mol Biol. 1983 Aug 15;168(3):505-23. (PMID: 6310122) ; Genetics. 1989 May;122(1):19-27. (PMID: 2659436) ; PLoS Genet. 2013;9(1):e1003208. (PMID: 23390378) ; Chromosoma. 1997 Jul;106(2):63-9. (PMID: 9215555) ; Mol Cell Biol. 2001 Mar;21(5):1819-27. (PMID: 11238918) ; Science. 2008 Aug 22;321(5892):1088-91. (PMID: 18719285) ; Nat Genet. 2023 Aug;55(8):1390-1399. (PMID: 37524789) ; Mol Cell. 2021 Apr 15;81(8):1816-1829.e5. (PMID: 33639094) ; Mol Cell Biol. 1988 May;8(5):2257-60. (PMID: 3290654) ; Curr Genet. 1991 Nov;20(5):411-5. (PMID: 1807832) ; EMBO J. 2006 Feb 22;25(4):857-67. (PMID: 16467853) ; PLoS Genet. 2009 Jun;5(6):e1000535. (PMID: 19557187) ; Annu Rev Biochem. 2006;75:111-35. (PMID: 16756487) ; Science. 1998 Oct 16;282(5388):493-6. (PMID: 9774280) ; Genetics. 1999 Aug;152(4):1531-41. (PMID: 10430581) ; Curr Biol. 2001 Jan 23;11(2):125-9. (PMID: 11231130) ; Cell. 1983 Jun;33(2):563-73. (PMID: 6345000) Grant Information: 2023YFA0913400 National Key Research and Development Program of China; 32150004 The National Natural Science Foundation of China Contributed Indexing: Keywords: S. cerevisiae; chromosome fusion; genetics; genomics; subtelomeric; telomere; yeast Substance Nomenclature: EC 2.7.7.49 (Telomerase) ; 0 (Saccharomyces cerevisiae Proteins) Entry Date(s): Date Created: 20240424 Date Completed: 20240424 Latest Revision: 20240426 Update Code: 20240426 PubMed Central ID: PMC11042809

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Elimination of subtelomeric repeat sequences exerts little effect on telomere essential functions in Saccharomyces cerevisiae .