Rosemary essential oil potentiates the antitumour activity of 5-fluorouracil in human colorectal carcinoma cells.

El Sala, AA ; Khawaja, G ; et al.

In: The Journal of pharmacy and pharmacology, Jg. 76 (2024-06-06), Heft 6, S. 691-700

academicJournal

Objectives: Improving response rates in colorectal cancer (CRC) is an urgent clinical need. This study aimed to explore the synergistic action of Lebanese rosemary essential oil (REO) and 5-fluorouracil (5-FU) in HCT116 CRC cells.

Methods: We tested the cell viability of monotherapy and combination therapy. The combination index was calculated using CompuSyn software to evaluate drug-drug interactions and the level of synergistic cytotoxicity. We also evaluated cell migration and cytopathology. Furthermore, cell apoptosis-related proteins (i.e. Bax and Bcl-2) were measured by Western blot analysis.

Key Findings: The REO/5-FU combination synergistically reduced cell viability, effectively decreased cell migration, and increased the Bax/Bcl-2 ratio in HCT116 cells. This triggered a proapoptotic morphology and initiated an apoptotic cascade in HCT116 cells, as indicated by a higher Bax/Bcl-2 ratio.

Conclusions: Our results provide evidence of the REO/5-FU combination as a better approach to improve 5-FU anticancer efficacy and allow the use of lower 5-FU doses due to the adjuvant effect of REO.

(© The Author(s) 2024. Published by Oxford University Press on behalf of the Royal Pharmaceutical Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Titel:	Rosemary essential oil potentiates the antitumour activity of 5-fluorouracil in human colorectal carcinoma cells.
Autor/in / Beteiligte Person:	El Sala, AA ; Khawaja, G ; Khalil, M ; Salam, SA
Zeitschrift:	The Journal of pharmacy and pharmacology, Jg. 76 (2024-06-06), Heft 6, S. 691-700
Veröffentlichung:	2021- : [Oxford] : Oxford University Press ; <i>Original Publication</i>: London : Pharmaceutical Society of Great Britain, 2024
Medientyp:	academicJournal
ISSN:	2042-7158 (electronic)
DOI:	10.1093/jpp/rgae022
Schlagwort:	Humans HCT116 Cells Cell Movement drug effects Antineoplastic Combined Chemotherapy Protocols pharmacology Rosmarinus chemistry Fluorouracil pharmacology Oils, Volatile pharmacology Colorectal Neoplasms drug therapy Colorectal Neoplasms pathology Drug Synergism Apoptosis drug effects Cell Survival drug effects Proto-Oncogene Proteins c-bcl-2 metabolism bcl-2-Associated X Protein metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [J Pharm Pharmacol] 2024 Jun 06; Vol. 76 (6), pp. 691-700. MeSH Terms: Fluorouracil* / pharmacology ; Oils, Volatile* / pharmacology ; Colorectal Neoplasms* / drug therapy ; Colorectal Neoplasms* / pathology ; Drug Synergism* ; Apoptosis* / drug effects ; Cell Survival* / drug effects ; Proto-Oncogene Proteins c-bcl-2* / metabolism ; bcl-2-Associated X Protein* / metabolism ; Humans ; HCT116 Cells ; Cell Movement / drug effects ; Antineoplastic Combined Chemotherapy Protocols / pharmacology ; Rosmarinus / chemistry Contributed Indexing: Keywords: 5-fluorouracil; HCT116 colorectal cancer cells; apoptosis; cell migration; rosemary essential oil; survival Substance Nomenclature: U3P01618RT (Fluorouracil) ; 0 (Oils, Volatile) ; 0 (Proto-Oncogene Proteins c-bcl-2) ; 0 (bcl-2-Associated X Protein) ; 8LGU7VM393 (rosemary oil) ; 0 (BCL2 protein, human) ; 0 (BAX protein, human) Entry Date(s): Date Created: 20240309 Date Completed: 20240606 Latest Revision: 20240606 Update Code: 20240606

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