Einzeltreffer — DigiBib

Neurogenins are proneural transcription factors required to specify neuronal identity. Their overexpression in human pluripotent stem cells rapidly produces cortical-like neurons with spiking activity and, because of this, they have been widely adopted for human neuron disease models. However, we do not fully understand the key downstream regulatory effectors responsible for driving neural differentiation. Here, using inducible expression of NEUROG1 and NEUROG2, we identify transcription factors (TFs) required for directed neuronal differentiation by combining expression and chromatin accessibility analyses with a pooled in vitro CRISPR-Cas9 screen targeting all ~1900 TFs in the human genome. The loss of one of these essential TFs (ZBTB18) yields few MAP2-positive neurons. Differentiated ZBTB18-null cells have radically altered gene expression, leading to cytoskeletal defects and stunted neurites and spines. In addition to identifying key downstream TFs for neuronal differentiation, our work develops an integrative multi-omics and TFome-wide perturbation platform to rapidly characterize essential TFs for the differentiation of any human cell type.

Titel:	Essential transcription factors for induced neuron differentiation.
Autor/in / Beteiligte Person:	Lu, C ; Garipler, G ; Dai, C ; Roush, T ; Salome-Correa, J ; Martin, A ; Liscovitch-Brauer, N ; Mazzoni, EO ; Sanjana, NE
Zeitschrift:	Nature communications, Jg. 14 (2023-12-15), Heft 1, S. 8362
Veröffentlichung:	[London] : Nature Pub. Group, 2023
Medientyp:	academicJournal
ISSN:	2041-1723 (electronic)
DOI:	10.1038/s41467-023-43602-7
Schlagwort:	Humans Basic Helix-Loop-Helix Transcription Factors metabolism Neurogenesis genetics Neurons metabolism Cell Differentiation genetics Transcription Factors genetics Transcription Factors metabolism Pluripotent Stem Cells metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Nat Commun] 2023 Dec 15; Vol. 14 (1), pp. 8362. <i>Date of Electronic Publication: </i>2023 Dec 15. MeSH Terms: Transcription Factors* / genetics ; Transcription Factors* / metabolism ; Pluripotent Stem Cells* / metabolism ; Humans ; Basic Helix-Loop-Helix Transcription Factors / metabolism ; Neurogenesis / genetics ; Neurons / metabolism ; Cell Differentiation / genetics References: Nature. 2017 May 11;545(7653):229-233. (PMID: 28445466) ; Front Mol Neurosci. 2021 Feb 15;14:642016. (PMID: 33658912) ; Development. 2018 Oct 1;145(19):. (PMID: 30201687) ; Nat Methods. 2017 Aug 31;14(9):831-832. (PMID: 28858339) ; Cell Death Differ. 2012 Apr;19(4):692-702. (PMID: 22095278) ; EMBO J. 2004 Jul 21;23(14):2892-902. (PMID: 15229646) ; Nat Methods. 2017 Oct;14(10):975-978. (PMID: 28825706) ; Genome Res. 2019 Mar;29(3):449-463. (PMID: 30696696) ; Development. 1997 Nov;124(22):4557-69. (PMID: 9409673) ; Cell Rep. 2018 May 22;23(8):2509-2523. (PMID: 29791859) ; Dev Cell. 2006 Dec;11(6):831-44. (PMID: 17141158) ; Nat Neurosci. 2019 Jun;22(6):897-908. (PMID: 31086315) ; Proc Natl Acad Sci U S A. 2008 Dec 23;105(51):20380-5. (PMID: 19091943) ; G3 (Bethesda). 2020 Jun 1;10(6):1949-1962. (PMID: 32273286) ; Eur J Neurosci. 2007 May;25(9):2591-603. (PMID: 17466019) ; Brain Res. 2019 Feb 15;1705:15-23. (PMID: 29501651) ; J Comp Neurol. 2008 Apr 1;507(4):1497-520. (PMID: 18205207) ; Clin Genet. 2017 May;91(5):697-707. (PMID: 27598823) ; Mol Syst Biol. 2014 Nov 17;10:760. (PMID: 25403753) ; Science. 2018 Oct 12;362(6411):176-180. (PMID: 30309944) ; Mol Cell Biol. 2008 Mar;28(5):1456-69. (PMID: 18160702) ; BMC Bioinformatics. 2009 Feb 03;10:48. (PMID: 19192299) ; Neuron. 2001 Sep 13;31(5):757-71. (PMID: 11567615) ; Development. 1999 Dec;126(23):5285-94. (PMID: 10556054) ; Cancer Res. 2010 Feb 1;70(3):1236-46. (PMID: 20103640) ; Neuroscience. 2013 Dec 3;253:256-73. (PMID: 23999125) ; J Comp Neurol. 2011 May 1;519(7):1355-70. (PMID: 21452201) ; Neuron. 2016 Jan 20;89(2):248-68. (PMID: 26796689) ; J Neurosci. 2012 Jun 6;32(23):7791-805. (PMID: 22674256) ; Dev Biol. 2011 Feb 1;350(1):198-207. (PMID: 21034732) ; Nat Rev Neurosci. 2002 Jul;3(7):517-30. (PMID: 12094208) ; Neuron. 2001 Feb;29(2):401-13. (PMID: 11239431) ; Nucleic Acids Res. 2020 Jan 8;48(D1):D87-D92. (PMID: 31701148) ; Hum Genet. 2017 Apr;136(4):463-479. (PMID: 28283832) ; Neuron. 2013 Jun 5;78(5):785-98. (PMID: 23764284) ; Dev Biol. 2004 Sep 15;273(2):373-89. (PMID: 15328020) ; BMC Biol. 2008 Mar 31;6:15. (PMID: 18377642) ; Cell. 2013 Oct 24;155(3):621-35. (PMID: 24243019) ; J Vis Exp. 2019 Mar 22;(145):. (PMID: 30958473) ; Cell. 2001 Feb 9;104(3):365-76. (PMID: 11239394) ; Stem Cell Res. 2019 Dec;41:101643. (PMID: 31707212) ; Mol Cell Biol. 2021 Jun 23;41(7):e0052620. (PMID: 33903225) ; Neuron. 2021 Sep 15;109(18):2847-2863.e11. (PMID: 34407390) ; Genes Dev. 2000 Jan 1;14(1):67-80. (PMID: 10640277) ; Neuron. 2005 Oct 6;48(1):45-62. (PMID: 16202708) ; G3 (Bethesda). 2020 Jan 7;10(1):143-150. (PMID: 31676507) ; Nat Biotechnol. 2021 Oct;39(10):1270-1277. (PMID: 33927415) ; Cell. 2010 Mar 5;140(5):744-52. (PMID: 20211142) ; Genome Res. 2003 Nov;13(11):2498-504. (PMID: 14597658) ; Dev Biol. 2009 Jul 15;331(2):140-51. (PMID: 19409883) ; FASEB J. 2019 Apr;33(4):5287-5299. (PMID: 30698461) ; J Neurochem. 2008 Sep;106(6):2515-23. (PMID: 18643788) ; Nat Methods. 2014 Aug;11(8):783-784. (PMID: 25075903) ; Cell. 2019 Feb 7;176(4):882-896.e18. (PMID: 30639098) ; Nat Methods. 2019 May;16(5):409-412. (PMID: 31011186) ; Nature. 2019 Jun;570(7762):523-527. (PMID: 31168097) ; Nat Neurosci. 2009 Dec;12(12):1524-33. (PMID: 19881504) ; EMBO J. 2007 Dec 12;26(24):5093-108. (PMID: 18007592) ; Genome Res. 2016 Dec;26(12):1742-1752. (PMID: 27852650) ; Nat Genet. 2021 Nov;53(11):1564-1576. (PMID: 34650237) ; Nature. 2022 Feb;602(7896):268-273. (PMID: 35110736) ; Cereb Cortex. 2014 Mar;24(3):754-72. (PMID: 23180754) ; J Neurosci. 2014 Jan 8;34(2):539-53. (PMID: 24403153) ; PLoS One. 2011;6(12):e27495. (PMID: 22194785) ; G3 (Bethesda). 2020 Sep 2;10(9):3071-3085. (PMID: 32601060) ; Cell. 1996 Oct 4;87(1):43-52. (PMID: 8858147) ; Cereb Cortex. 2006 Jul;16 Suppl 1:i138-51. (PMID: 16766700) ; Curr Opin Neurobiol. 2002 Feb;12(1):26-34. (PMID: 11861161) ; Nat Rev Neurosci. 2016 Jul;17(7):424-37. (PMID: 27194476) ; EMBO J. 2001 May 15;20(10):2536-44. (PMID: 11350943) ; Cell. 2015 Mar 12;160(6):1246-60. (PMID: 25748654) ; J Neurosci. 2011 Jul 27;31(30):10859-71. (PMID: 21795538) ; Nature. 2020 Jun;582(7812):416-420. (PMID: 32499641) Grant Information: R01 NS124920 United States NS NINDS NIH HHS; R00 HG008171 United States HG NHGRI NIH HHS; R01 CA279135 United States CA NCI NIH HHS; R01 HD079682 United States HD NICHD NIH HHS; R01 GM138876 United States GM NIGMS NIH HHS; R01 HG012790 United States HG NHGRI NIH HHS; R01 CA218668 United States CA NCI NIH HHS; R01 GM138635 United States GM NIGMS NIH HHS; DP2 HG010099 United States HG NHGRI NIH HHS; R01 AI176601 United States AI NIAID NIH HHS Substance Nomenclature: 0 (Transcription Factors) ; 0 (Basic Helix-Loop-Helix Transcription Factors) Entry Date(s): Date Created: 20231215 Date Completed: 20231218 Latest Revision: 20240131 Update Code: 20240131 PubMed Central ID: PMC10724217

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Essential transcription factors for induced neuron differentiation.