Einzeltreffer — DigiBib

Connections between the nucleus and the cytoskeleton are important for positioning and division of the nucleus. In most eukaryotes, the linker of nucleoskeleton and cytoskeleton (LINC) complex spans the outer and inner nuclear membranes and connects the nucleus to the cytoskeleton. In opisthokonts, it is composed of Klarsicht, ANC-1 and Syne homology (KASH) domain proteins and Sad1 and UNC-84 (SUN) domain proteins. Given that the nucleus is positioned at the posterior pole of Toxoplasma gondii, we speculated that apicomplexan parasites must have a similar mechanism that integrates the nucleus and the cytoskeleton. Here, we identified three UNC family proteins in the genome of the apicomplexan parasite T. gondii. Whereas the UNC-50 protein TgUNC1 localised to the Golgi and appeared to be not essential for the parasite, the SUN domain protein TgSLP2 showed a diffuse pattern throughout the parasite. The second SUN domain protein, TgSLP1, was expressed in a cell cycle-dependent manner and was localised close to the mitotic spindle and, more detailed, at the kinetochore. We demonstrate that conditional knockout of TgSLP1 leads to failure of nuclear division and loss of centrocone integrity.

Competing Interests: Competing interests The authors declare no competing or financial interests.

Titel:	The SUN-like protein TgSLP1 is essential for nuclear division in the apicomplexan parasite Toxoplasma gondii.
Autor/in / Beteiligte Person:	Wagner, M ; Song, Y ; Jiménez-Ruiz, E ; Härtle, S ; Meissner, M
Zeitschrift:	Journal of cell science, Jg. 136 (2023-11-01), Heft 21
Veröffentlichung:	Cambridge : Company of Biologists ; <i>Original Publication</i>: London., 2023
Medientyp:	academicJournal
ISSN:	1477-9137 (electronic)
DOI:	10.1242/jcs.260337
Schlagwort:	Animals Nuclear Envelope metabolism Spindle Apparatus Cell Nucleus Division Parasites Toxoplasma genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Cell Sci] 2023 Nov 01; Vol. 136 (21). <i>Date of Electronic Publication: </i>2023 Oct 30. MeSH Terms: Parasites* ; Toxoplasma* / genetics ; Animals ; Nuclear Envelope / metabolism ; Spindle Apparatus ; Cell Nucleus Division References: J Cell Biol. 2006 Jan 2;172(1):41-53. (PMID: 16380439) ; J Cell Sci. 2006 Jun 1;119(Pt 11):2236-45. (PMID: 16684814) ; J Cell Sci. 2008 May 1;121(Pt 9):1559-68. (PMID: 18411248) ; Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) ; Elife. 2019 Jul 19;8:. (PMID: 31322501) ; Nat Biotechnol. 2018 Oct;36(9):880-887. (PMID: 30125270) ; Science. 2002 Oct 11;298(5592):406-9. (PMID: 12169658) ; J Cell Biol. 2012 Jan 23;196(2):203-11. (PMID: 22270916) ; Cell Host Microbe. 2020 Nov 11;28(5):752-766.e9. (PMID: 33053376) ; J Cell Biol. 2009 Jul 27;186(2):229-41. (PMID: 19635841) ; Development. 1999 Jun;126(14):3171-81. (PMID: 10375507) ; Cell. 2003 Dec 26;115(7):825-36. (PMID: 14697201) ; J Cell Biol. 2018 Jun 4;217(6):2005-2018. (PMID: 29650775) ; J Cell Biol. 2002 Mar 18;156(6):1039-50. (PMID: 11901169) ; EMBO Rep. 2019 Dec 5;20(12):e48896. (PMID: 31584242) ; Mol Biochem Parasitol. 2001 Jul;115(2):165-75. (PMID: 11420103) ; J Cell Sci. 2005 Aug 1;118(Pt 15):3419-30. (PMID: 16079285) ; Front Cell Infect Microbiol. 2021 Apr 12;11:670049. (PMID: 33912479) ; mSphere. 2018 Jul 5;3(4):. (PMID: 29976642) ; PLoS Biol. 2015 Mar 03;13(3):e1002093. (PMID: 25734885) ; Genes Dev. 2009 Apr 15;23(8):912-27. (PMID: 19390086) ; Bioinformatics. 2015 Oct 15;31(20):3359-61. (PMID: 26069263) ; Curr Opin Cell Biol. 2016 Jun;40:32-40. (PMID: 26895141) ; Cell Microbiol. 2014 Jan;16(1):78-94. (PMID: 24015880) ; Bioinformatics. 2014 May 1;30(9):1236-40. (PMID: 24451626) ; Curr Genet. 2017 Dec;63(6):1037-1052. (PMID: 28493118) ; Neuron. 2009 Oct 29;64(2):173-87. (PMID: 19874786) ; Cell Microbiol. 2016 Feb;18(2):151-67. (PMID: 26270241) ; Nat Protoc. 2018 Jan;13(1):307-323. (PMID: 29323662) ; J Cell Biol. 2019 May 6;218(5):1478-1490. (PMID: 30862629) ; mBio. 2017 May 2;8(3):. (PMID: 28465425) ; Microb Genom. 2015 Oct 30;1(4):e000033. (PMID: 28348817) ; PLoS One. 2015 Jun 19;10(6):e0130356. (PMID: 26090798) ; Nucleic Acids Res. 2008 Jan;36(Database issue):D553-6. (PMID: 18003657) ; Trends Parasitol. 2018 Sep;34(9):759-771. (PMID: 30078701) ; Mol Cell Biol. 2006 May;26(10):3738-51. (PMID: 16648470) ; J Cell Sci. 2002 Aug 1;115(Pt 15):3207-22. (PMID: 12118075) ; J Cell Sci. 2016 Oct 1;129(19):3523-3531. (PMID: 27591260) ; Mol Biochem Parasitol. 2008 Jan;157(1):22-31. (PMID: 17976834) ; J Cell Sci. 1998 Mar;111 ( Pt 6):701-12. (PMID: 9471999) ; Eukaryot Cell. 2005 Feb;4(2):432-42. (PMID: 15701805) ; PLoS One. 2010 Aug 19;5(8):e12302. (PMID: 20808817) ; Cell Cycle. 2015;14(14):2200-5. (PMID: 26083340) ; J Cell Biol. 2006 Aug 28;174(5):665-75. (PMID: 16923827) ; mBio. 2022 Oct 26;13(5):e0185922. (PMID: 36069445) ; Nat Methods. 2013 Feb;10(2):125-7. (PMID: 23263690) ; PLoS One. 2012;7(3):e32671. (PMID: 22427862) ; Curr Top Dev Biol. 2014;109:287-321. (PMID: 24947240) ; Cell. 2009 Nov 25;139(5):907-19. (PMID: 19913287) ; J Cell Biol. 1999 Jun 14;145(6):1233-49. (PMID: 10366596) ; Curr Opin Cell Biol. 2013 Feb;25(1):57-62. (PMID: 23149102) ; J Cell Biol. 2010 Oct 4;191(1):115-28. (PMID: 20921138) ; iScience. 2021 Nov 26;24(12):103514. (PMID: 34950858) ; J Microsc. 2006 Dec;224(Pt 3):213-32. (PMID: 17210054) ; Nucleus. 2013 May-Jun;4(3):206-15. (PMID: 23680964) ; J Exp Bot. 2014 Dec;65(22):6499-512. (PMID: 25217773) ; J Cell Biol. 2010 May 3;189(3):511-25. (PMID: 20440000) ; FEMS Microbiol Lett. 2000 Apr 1;185(1):79-82. (PMID: 10731610) ; Elife. 2017 Mar 21;6:. (PMID: 28322189) ; Mol Biol Cell. 2004 Feb;15(2):600-10. (PMID: 14617811) ; Development. 2001 Dec;128(24):5039-50. (PMID: 11748140) ; Nat Rev Microbiol. 2014 Feb;12(2):125-36. (PMID: 24384598) ; Cell. 2016 Sep 8;166(6):1423-1435.e12. (PMID: 27594426) ; Mol Biol Cell. 2016 Feb 1;27(3):549-71. (PMID: 26680740) ; Bio Protoc. 2018 Feb 20;8(4):. (PMID: 29644255) Contributed Indexing: Keywords: Toxoplasma; LINC; Parasite; Replication; SUN protein Entry Date(s): Date Created: 20231010 Date Completed: 20231031 Latest Revision: 20231115 Update Code: 20231215 PubMed Central ID: PMC10629696

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The SUN-like protein TgSLP1 is essential for nuclear division in the apicomplexan parasite Toxoplasma gondii.