Einzeltreffer — DigiBib

Plasmodium parasites, the eukaryotic pathogens that cause malaria, feature three distinct invasive forms tailored to the host environment they must navigate and invade for life cycle progression. One conserved feature of these invasive forms is the micronemes, apically oriented secretory organelles involved in egress, motility, adhesion, and invasion. Here we investigate the role of GPI-anchored micronemal antigen (GAMA), which shows a micronemal localization in all zoite forms of the rodent-infecting species Plasmodium berghei. ∆GAMA parasites are severely defective for invasion of the mosquito midgut. Once formed, oocysts develop normally, however, sporozoites are unable to egress and exhibit defective motility. Epitope-tagging of GAMA revealed tight temporal expression late during sporogony and showed that GAMA is shed during sporozoite gliding motility in a similar manner to circumsporozoite protein. Complementation of P. berghei knockout parasites with full-length P. falciparum GAMA partially restored infectivity to mosquitoes, indicating conservation of function across Plasmodium species. A suite of parasites with GAMA expressed under the promoters of CTRP, CAP380, and TRAP, further confirmed the involvement of GAMA in midgut infection, motility, and vertebrate infection. These data show GAMA's involvement in sporozoite motility, egress, and invasion, implicating GAMA as a regulator of microneme function.

Titel:	Plasmodium GPI-anchored micronemal antigen is essential for parasite transmission through the mosquito host.
Autor/in / Beteiligte Person:	Jennison, C ; Armstrong, JM ; Dankwa, DA ; Hertoghs, N ; Kumar, S ; Abatiyow, BA ; Naung, M ; Minkah, NK ; Swearingen, KE ; Moritz, R ; Barry, AE ; Kappe, SHI ; Vaughan, AM
Zeitschrift:	Molecular microbiology, Jg. 121 (2024-03-01), Heft 3, S. 394-412
Veröffentlichung:	Oxford, OX ; Boston, MA : Blackwell Scientific Publications, c1987-, 2024
Medientyp:	academicJournal
ISSN:	1365-2958 (electronic)
DOI:	10.1111/mmi.15078
Schlagwort:	Animals Protozoan Proteins genetics Protozoan Proteins metabolism Oocysts Plasmodium berghei genetics Plasmodium berghei metabolism Sporozoites metabolism Culicidae metabolism Culicidae parasitology Parasites metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural Language: English [Mol Microbiol] 2024 Mar; Vol. 121 (3), pp. 394-412. <i>Date of Electronic Publication: </i>2023 Jun 14. MeSH Terms: Culicidae* / metabolism ; Culicidae* / parasitology ; Parasites* / metabolism ; Animals ; Protozoan Proteins / genetics ; Protozoan Proteins / metabolism ; Oocysts ; Plasmodium berghei / genetics ; Plasmodium berghei / metabolism ; Sporozoites / metabolism References: Mol Microbiol. 2018 Aug;109(4):458-473. (PMID: 29873127) ; Mol Cell Proteomics. 2006 Jul;5(7):1286-99. (PMID: 16603573) ; Cell Host Microbe. 2017 Aug 9;22(2):232-245. (PMID: 28799908) ; Elife. 2016 Dec 01;5:. (PMID: 27906127) ; PLoS Pathog. 2013 Mar;9(3):e1003213. (PMID: 23505371) ; Proc Natl Acad Sci U S A. 2021 Nov 30;118(48):. (PMID: 34819379) ; J Biol Chem. 2004 Mar 5;279(10):9490-6. (PMID: 14676185) ; Pathogens. 2019 Apr 07;8(2):. (PMID: 30959972) ; Sci Rep. 2021 Feb 4;11(1):3090. (PMID: 33542254) ; J Cell Biol. 1999 Nov 29;147(5):937-44. (PMID: 10579715) ; Int J Parasitol. 2009 Mar;39(4):489-96. (PMID: 19000911) ; Cell Host Microbe. 2015 Nov 11;18(5):593-603. (PMID: 26607162) ; Front Cell Infect Microbiol. 2018 Nov 27;8:413. (PMID: 30547015) ; Cell Microbiol. 2008 Jun;10(6):1304-12. (PMID: 18248630) ; mBio. 2014 Jul 01;5(4):e01414-14. (PMID: 24987097) ; Methods Mol Biol. 2013;923:51-72. (PMID: 22990771) ; PLoS Pathog. 2015 May 27;11(5):e1004905. (PMID: 26018192) ; PLoS Pathog. 2012;8(7):e1002725. (PMID: 22911675) ; J Exp Med. 2005 Jul 18;202(2):225-30. (PMID: 16027235) ; Mol Cell Proteomics. 2013 May;12(5):1127-43. (PMID: 23325771) ; Cell. 1997 Aug 8;90(3):511-22. (PMID: 9267031) ; J Parasitol. 1975 Feb;61(1):43-50. (PMID: 1090717) ; PLoS One. 2011;6(12):e29289. (PMID: 22216235) ; Nature. 2014 Oct 2;514(7520):117-21. (PMID: 25119046) ; PLoS One. 2014 Dec 01;9(12):e113923. (PMID: 25438048) ; Malar J. 2018 Apr 2;17(1):135. (PMID: 29609625) ; Infect Immun. 2000 Nov;68(11):6461-5. (PMID: 11035760) ; EMBO J. 1995 Aug 15;14(16):3883-94. (PMID: 7664729) ; Proc Natl Acad Sci U S A. 2021 Mar 9;118(10):. (PMID: 33653959) ; J Biol Chem. 2005 Feb 25;280(8):6752-60. (PMID: 15590623) ; Biotechnol J. 2009 Jun;4(6):903-13. (PMID: 19455538) ; Cold Spring Harb Perspect Med. 2017 Jun 1;7(6):. (PMID: 28242785) ; Mol Microbiol. 2009 Mar;71(6):1402-14. (PMID: 19220746) ; Int J Parasitol. 2001 Oct;31(12):1293-302. (PMID: 11566297) ; J Proteome Res. 2019 Sep 6;18(9):3404-3418. (PMID: 31335145) ; Cell Microbiol. 2003 Sep;5(9):613-24. (PMID: 12925131) ; PLoS Negl Trop Dis. 2013 Oct 31;7(10):e2506. (PMID: 24205419) ; EMBO J. 1999 Nov 15;18(22):6221-7. (PMID: 10562534) ; Parasitology. 2008 Jan;135(Pt 1):1-12. (PMID: 17908361) ; PLoS Pathog. 2016 Apr 29;12(4):e1005606. (PMID: 27128092) ; Science. 2001 Jan 5;291(5501):141-4. (PMID: 11141568) ; Nat Protoc. 2008;3(6):1101-8. (PMID: 18546601) ; Cell Microbiol. 2004 Jul;6(7):671-85. (PMID: 15186403) ; Elife. 2017 Jan 24;6:. (PMID: 28115054) ; Cell Host Microbe. 2011 Jan 20;9(1):9-20. (PMID: 21238943) ; Exp Parasitol. 2007 Aug;116(4):504-8. (PMID: 17367780) ; Cell. 2007 Dec 14;131(6):1072-83. (PMID: 18083098) ; Eukaryot Cell. 2009 Dec;8(12):1869-79. (PMID: 19820120) ; Curr Opin Cell Biol. 2016 Aug;41:18-24. (PMID: 27019341) ; Cell Host Microbe. 2009 Dec 17;6(6):551-62. (PMID: 20006843) ; Proc Natl Acad Sci U S A. 1999 Nov 23;96(24):14061-6. (PMID: 10570198) ; Science. 2019 Aug 23;365(6455):. (PMID: 31439762) ; Infect Immun. 2014 Nov;82(11):4643-53. (PMID: 25156733) ; Mol Microbiol. 2006 Mar;59(5):1369-79. (PMID: 16468982) ; Mol Microbiol. 2008 Oct;70(1):209-20. (PMID: 18761621) ; Cell Microbiol. 2016 Jul;18(7):905-18. (PMID: 27111866) ; Proc Biol Sci. 1990 Dec 22;242(1305):205-16. (PMID: 1983036) ; J Protozool. 1991 Jul-Aug;38(4):411-21. (PMID: 1787427) ; ACS Nano. 2012 Jun 26;6(6):4648-62. (PMID: 22568891) ; Eukaryot Cell. 2008 Jun;7(6):1062-70. (PMID: 18441124) ; J Exp Med. 1990 Jul 1;172(1):379-82. (PMID: 1694225) ; Cell Rep. 2017 Mar 28;18(13):3105-3116. (PMID: 28355563) ; Parasit Vectors. 2008 Sep 22;1(1):33. (PMID: 18808667) ; Science. 2018 May 4;360(6388):. (PMID: 29724925) ; Cell Host Microbe. 2019 Nov 13;26(5):579-590.e5. (PMID: 31726028) ; Cell Microbiol. 2019 May;21(5):e13018. (PMID: 30791192) ; Mol Microbiol. 2010 Feb;75(4):854-63. (PMID: 20025671) ; PLoS Pathog. 2009 Sep;5(9):e1000599. (PMID: 19779564) ; Infect Immun. 2011 Nov;79(11):4523-32. (PMID: 21896773) ; Subcell Biochem. 2008;47:33-45. (PMID: 18512339) ; Mol Biochem Parasitol. 2006 Jan;145(1):60-70. (PMID: 16242190) ; Nat Protoc. 2006;1(1):346-56. (PMID: 17406255) ; Cell Host Microbe. 2020 Jun 10;27(6):950-962.e7. (PMID: 32396839) ; BMC Genomics. 2008 Feb 07;9:70. (PMID: 18257930) ; mSphere. 2016 Feb 10;1(1):. (PMID: 27303694) ; J Biol Chem. 2003 Jun 27;278(26):23890-8. (PMID: 12686561) ; Malar J. 2012 Dec 17;11:421. (PMID: 23244590) Grant Information: K25 AI119229 United States AI NIAID NIH HHS; R21AI146391 United States NH NIH HHS; R21 AI146391 United States AI NIAID NIH HHS; R01 AI148489 United States AI NIAID NIH HHS; K25AI119229 United States NH NIH HHS; R01 GM087221 United States GM NIGMS NIH HHS; R01AI148489 United States NH NIH HHS; R01GM087221 United States NH NIH HHS Contributed Indexing: Keywords: Plasmodium berghei; malaria; microneme; mosquito stage; sporozoite Substance Nomenclature: 0 (Protozoan Proteins) Entry Date(s): Date Created: 20230614 Date Completed: 20240312 Latest Revision: 20240509 Update Code: 20240509 PubMed Central ID: PMC11076100

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

Plasmodium GPI-anchored micronemal antigen is essential for parasite transmission through the mosquito host.