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Many animals use Earth's magnetic field (also known as the geomagnetic field) for navigation 1 . The favoured mechanism for magnetosensitivity involves a blue-light-activated electron-transfer reaction between flavin adenine dinucleotide (FAD) and a chain of tryptophan residues within the photoreceptor protein CRYPTOCHROME (CRY). The spin-state of the resultant radical pair, and therefore the concentration of CRY in its active state, is influenced by the geomagnetic field 2 . However, the canonical CRY-centric radical-pair mechanism does not explain many physiological and behavioural observations 2-8 . Here, using electrophysiology and behavioural analyses, we assay magnetic-field responses at the single-neuron and organismal levels. We show that the 52 C-terminal amino acid residues of Drosophila melanogaster CRY, lacking the canonical FAD-binding domain and tryptophan chain, are sufficient to facilitate magnetoreception. We also show that increasing intracellular FAD potentiates both blue-light-induced and magnetic-field-dependent effects on the activity mediated by the C terminus. High levels of FAD alone are sufficient to cause blue-light neuronal sensitivity and, notably, the potentiation of this response in the co-presence of a magnetic field. These results reveal the essential components of a primary magnetoreceptor in flies, providing strong evidence that non-canonical (that is, non-CRY-dependent) radical pairs can elicit magnetic-field responses in cells.

Titel:	Essential elements of radical pair magnetosensitivity in Drosophila.
Autor/in / Beteiligte Person:	Bradlaugh, AA ; Fedele, G ; Munro, AL ; Hansen, CN ; Hares, JM ; Patel, S ; Kyriacou, CP ; Jones, AR ; Rosato, E ; Baines, RA
Link:	View record from Nature Publishing (Volltext)
Zeitschrift:	Nature, Jg. 615 (2023-03-01), Heft 7950, S. 111-116
Veröffentlichung:	Basingstoke : Nature Publishing Group ; <i>Original Publication</i>: London, Macmillan Journals ltd., 2023
Medientyp:	academicJournal
ISSN:	1476-4687 (electronic)
DOI:	10.1038/s41586-023-05735-z
Schlagwort:	Animals Flavin-Adenine Dinucleotide metabolism Tryptophan metabolism Electrophysiology Behavior, Animal Single-Cell Analysis Neurons cytology Neurons metabolism Cryptochromes chemistry Cryptochromes metabolism Drosophila melanogaster chemistry Drosophila melanogaster cytology Drosophila melanogaster metabolism Drosophila melanogaster physiology Magnetic Fields
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Essential elements of radical pair magnetosensitivity in Drosophila.