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Background: During early embryonic development, the cell adhesion molecule E-cadherin encoded by the Cdh1 gene plays a vital role in providing proper cell-cell adhesion, ensuring an undifferentiated state critical for maintaining the pluripotency for the development of the preimplantation embryo. The transcriptional regulation of Cdh1 gained attention recently but is not yet fully understood. In a previous study, our team established a correlation between Elf3 and Cdh1 expression and showed its importance in the regulation of MET.

Methods and Results: Here, the regulation of Cdh1 by Ets transcription factors in early embryogenesis was investigated. A loss-of-function approach was used to study the effect of Elf5 loss on Cdh1 gene expression by small interfering RNAs in fertilized oocytes. Changes in gene expression were measured by qPCR analysis, and developing embryos were visualized by microscopy. Loss of Elf5 arrested the embryos at the 2-cell stage, accompanied by a significant downregulation of Cdh1 expression.

Conclusion: The findings presented here illustrate the role of ELF5 in preimplantation development and in regulating the expression of Cdh1. The maintenance of the ELF5 and Cdh1 regulatory node proved essential for the proper development of the early mouse embryos, which is in agreement with the critical role of Elf5 and Cdh1 genes in regulating the early events during embryogenesis.

Titel:	The transcription factor ELF5 is essential for early preimplantation development.
Autor/in / Beteiligte Person:	Alotaibi, H
Zeitschrift:	Molecular biology reports, Jg. 50 (2023-03-01), Heft 3, S. 2119-2125
Veröffentlichung:	Dordrecht, Boston, Reidel., 2023
Medientyp:	academicJournal
ISSN:	1573-4978 (electronic)
DOI:	10.1007/s11033-022-08217-z
Schlagwort:	Animals Female Mice Pregnancy Blastocyst metabolism Gene Expression Regulation Embryonic Development genetics Transcription Factors metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Mol Biol Rep] 2023 Mar; Vol. 50 (3), pp. 2119-2125. <i>Date of Electronic Publication: </i>2022 Dec 21. MeSH Terms: Embryonic Development* / genetics ; Transcription Factors* / metabolism ; Animals ; Female ; Mice ; Pregnancy ; Blastocyst / metabolism ; Gene Expression Regulation References: Schultz RM (1993) Regulation of zygotic gene activation in the mouse. BioEssays 15(8):531–538. (PMID: 10.1002/bies.9501508068135766) ; Jukam D, Shariati SAM, Skotheim JM (2017) Zygotic genome activation in vertebrates. Dev Cell 42(4):316–332. (PMID: 10.1016/j.devcel.2017.07.026288299425714289) ; Shi L, Wu J (2009) Epigenetic regulation in mammalian preimplantation embryo development. Reprod Biol Endocrinol 7:59. 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(PMID: 10.1371/journal.pone.0117510256297354309403) Grant Information: Izmir Biomedicine and Genome Center Izmir Biomedicine and Genome Center; Max-Planck-Society Max-Planck-Society Contributed Indexing: Keywords: Cdh1; Elf3; Elf5; Preimplantation development Substance Nomenclature: 0 (Transcription Factors) ; 0 (Elf5 protein, mouse) Entry Date(s): Date Created: 20221221 Date Completed: 20230317 Latest Revision: 20230317 Update Code: 20240513

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The transcription factor ELF5 is essential for early preimplantation development.