In Vitro and In Silico Evaluation of ACE2 and LOX Inhibitory Activity of Origanum Essential Oils and Carvacrol.
In: Planta medica, Jg. 89 (2023-07-01), Heft 8, S. 790-799
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Zugriff:
Origanum spp. are used both for culinary purposes and for their biological activities. In this study, commercial Origanum majorana, Origanum minutiflorum, Origanum vulgare , and Origanum onites essential oils and their prominent constituent carvacrol were evaluated for their in vitro and in silico angiotensin-converting enzyme 2 and lipoxygenase enzyme inhibitory potentials. The essential oils were analysed by gas chromatography-flame ionisation detection and gas chromatography-mass spectrometry, where carvacrol was identified as the major component (62 - 81%), confirming the quality. In vitro enzyme inhibition assays were conducted both with the essential oils (20 µg/mL) and with carvacrol (5 µg/mL). The comparative values of angiotensin-converting enzyme 2 percent inhibition for O. majorana, O. minutiflorum, O. vulgare , and O. onites essential oils were determined as 85.5, 79.1, 74.3, and 42.8%, respectively. As a result of the enzyme assays, carvacrol showed 90.7% in vitro angiotensin-converting enzyme 2 inhibitory activity. The in vitro lipoxygenase inhibition of the essential oils (in the same order) was 89.4, 78.9, 81.1, and 73.5%, respectively, where carvacrol showed 74.8% inhibition. In addition, protein-ligand docking and interaction profiling was used to gain structural and mechanistic insights into the angiotensin-converting enzyme 2 and lipoxygenase inhibitory potentials of major Origanum essential oil constituents. The in silico findings agreed with the significant enzyme inhibition activity observed in vitro . Further in vivo studies are suggested to confirm the safety and efficacy of the oils.
Competing Interests: The authors declare that they have no conflict of interest.
(Thieme. All rights reserved.)
Titel: |
In Vitro and In Silico Evaluation of ACE2 and LOX Inhibitory Activity of Origanum Essential Oils and Carvacrol.
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Autor/in / Beteiligte Person: | Demirci, F ; Teralı, K ; Karadağ, AE ; Biltekin, SN ; Ak Sakallı, E ; Demirci, B ; Koşar, M ; Başer, KHC |
Zeitschrift: | Planta medica, Jg. 89 (2023-07-01), Heft 8, S. 790-799 |
Veröffentlichung: | Stuttgart ; New York : George Thieme ; <i>Original Publication</i>: Stuttgart, Hippokrates Verlag., 2023 |
Medientyp: | academicJournal |
ISSN: | 1439-0221 (electronic) |
DOI: | 10.1055/a-1828-2479 |
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