Einzeltreffer — DigiBib

The organization of genomic DNA into defined nucleosomes has long been viewed as a hallmark of eukaryotes. This paradigm has been challenged by the identification of "minimalist" histones in archaea and more recently by the discovery of genes that encode fused remote homologs of the four eukaryotic histones in Marseilleviridae, a subfamily of giant viruses that infect amoebae. We demonstrate that viral doublet histones are essential for viral infectivity, localize to cytoplasmic viral factories after virus infection, and ultimately are found in the mature virions. Cryogenic electron microscopy (cryo-EM) structures of viral nucleosome-like particles show strong similarities to eukaryotic nucleosomes despite the limited sequence identify. The unique connectors that link the histone chains contribute to the observed instability of viral nucleosomes, and some histone tails assume structural roles. Our results further expand the range of "organisms" that require nucleosomes and suggest a specialized function of histones in the biology of these unusual viruses.

Competing Interests: Declaration of interests The authors declare no competing interests.

Titel:	Virus-encoded histone doublets are essential and form nucleosome-like structures.
Autor/in / Beteiligte Person:	Liu, Y ; Bisio, H ; Toner, CM ; Jeudy, S ; Philippe, N ; Zhou, K ; Bowerman, S ; White, A ; Edwards, G ; Abergel, C ; Luger, K
Zeitschrift:	Cell, Jg. 184 (2021-08-05), Heft 16, S. 4237-4250.e19
Veröffentlichung:	Cambridge, Ma : Cell Press ; <i>Original Publication</i>: Cambridge, MIT Press., 2021
Medientyp:	academicJournal
ISSN:	1097-4172 (electronic)
DOI:	10.1016/j.cell.2021.06.032
Schlagwort:	Amoeba virology Fluorescent Dyes metabolism Histones chemistry Models, Molecular Proteomics Virion metabolism DNA Viruses metabolism Histones metabolism Nucleosomes metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Language: English [Cell] 2021 Aug 05; Vol. 184 (16), pp. 4237-4250.e19. <i>Date of Electronic Publication: </i>2021 Jul 22. MeSH Terms: DNA Viruses / metabolism ; Histones / metabolism ; Nucleosomes / *metabolism ; Amoeba / virology ; Fluorescent Dyes / metabolism ; Histones / chemistry ; Models, Molecular ; Proteomics ; Virion / metabolism Comments: Comment in: Mol Cell. 2021 Sep 2;81(17):3447-3448. (PMID: 34478653) References: Protein Expr Purif. 2010 Mar;70(1):95-100. (PMID: 19836453) ; Elife. 2017 Dec 27;6:. (PMID: 29280735) ; J Mol Graph. 1996 Feb;14(1):33-8, 27-8. (PMID: 8744570) ; EMBO J. 2021 Jan 4;40(1):e105907. (PMID: 33073403) ; Proc Natl Acad Sci U S A. 2017 Jan 10;114(2):334-339. (PMID: 28028239) ; Genetics. 2015 Jul;200(3):795-806. (PMID: 25971662) ; Methods Enzymol. 2004;375:23-44. (PMID: 14870657) ; Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32. (PMID: 15572765) ; Commun Biol. 2020 Nov 3;3(1):643. (PMID: 33144673) ; Front Microbiol. 2020 Apr 14;11:651. (PMID: 32390970) ; J Mol Biol. 2021 Mar 19;433(6):166684. (PMID: 33098859) ; Curr Opin Genet Dev. 1998 Apr;8(2):140-6. (PMID: 9610403) ; Sci Rep. 2016 Jan 11;6:19122. (PMID: 26750377) ; Curr Protoc Bioinformatics. 2016 Jun 20;54:5.6.1-5.6.37. (PMID: 27322406) ; Nat Methods. 2008 Jan;5(1):53-5. (PMID: 18157137) ; J Mol Biol. 2018 Jul 20;430(15):2237-2243. (PMID: 29258817) ; Biopolymers. 2012 Sep;97(9):678-86. (PMID: 22696404) ; J Comput Chem. 2004 Oct;25(13):1605-12. (PMID: 15264254) ; J Mol Biol. 1993 Dec 5;234(3):779-815. (PMID: 8254673) ; J Struct Biol. 2016 Jan;193(1):1-12. (PMID: 26592709) ; Nucleic Acids Res. 2012 Jul;40(13):6338-52. (PMID: 22453276) ; Electrophoresis. 2009 Jun;30 Suppl 1:S162-73. (PMID: 19517507) ; Virology. 2018 Mar;516:239-245. (PMID: 29407382) ; Proc Natl Acad Sci U S A. 1975 Aug;72(8):2895-9. (PMID: 171647) ; Environ Microbiol. 2011 Jun;13(6):1454-66. (PMID: 21392201) ; Life Sci Alliance. 2018 Jul 10;1(4):e201800107. (PMID: 30456370) ; J Chem Theory Comput. 2013 Jul 9;9(7):3084-95. (PMID: 26583988) ; BMC Bioinformatics. 2011 Aug 04;12:323. (PMID: 21816040) ; Science. 2017 Aug 11;357(6351):609-612. (PMID: 28798133) ; Elife. 2021 Mar 02;10:. (PMID: 33650488) ; Bioinformatics. 2010 Jan 1;26(1):139-40. (PMID: 19910308) ; Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501. (PMID: 20383002) ; Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21848-53. (PMID: 20007369) ; Nat Struct Mol Biol. 2021 May;28(5):413-417. (PMID: 33927388) ; Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286) ; Methods. 2016 May 1;100:50-60. (PMID: 26804562) ; Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21. (PMID: 20124702) ; Viruses. 2020 Nov 07;12(11):. (PMID: 33171839) ; Anal Chem. 2014 Aug 5;86(15):7688-95. (PMID: 25010012) ; Nucleic Acids Res. 2017 Jan 4;45(D1):D313-D319. (PMID: 27899672) ; Macromol Biosci. 2010 Jul 7;10(7):746-53. (PMID: 20480513) ; Nucleic Acids Res. 2018 Jul 2;46(W1):W296-W303. (PMID: 29788355) ; J Mol Evol. 2001 Sep;53(3):251-6. (PMID: 11523012) ; Nat Rev Genet. 2019 May;20(5):283-297. (PMID: 30886348) ; J Cell Physiol. 2008 Aug;216(2):295-302. (PMID: 18314879) ; Elife. 2018 Apr 12;7:. (PMID: 29648537) ; Epigenetics Chromatin. 2017 Nov 28;10(1):55. (PMID: 29179736) ; J Mol Biol. 2003 Mar 14;327(1):85-96. (PMID: 12614610) ; Nat Methods. 2017 Apr;14(4):331-332. (PMID: 28250466) ; Nature. 1997 Sep 18;389(6648):251-60. (PMID: 9305837) ; Curr Protoc Mol Biol. 2020 Dec;133(1):e131. (PMID: 33351266) ; J Mol Biol. 2010 Oct 15;403(1):1-10. (PMID: 20800598) ; J Comput Chem. 2013 Sep 30;34(25):2135-45. (PMID: 23832629) ; Nat Commun. 2017 Apr 21;8:15087. (PMID: 28429720) ; PLoS One. 2015 Feb 24;10(2):e0117471. (PMID: 25710170) ; Curr Opin Struct Biol. 1998 Feb;8(1):33-40. (PMID: 9519294) ; Biophys J. 2014 Apr 15;106(8):1741-50. (PMID: 24739173) Grant Information: F32 GM137496 United States GM NIGMS NIH HHS; United States HHMI Howard Hughes Medical Institute Contributed Indexing: Keywords: Analytical Ultracentrifugation; KO fitness impact; Melbournevirus genetics; NCLDV; acidic patch; cryo-EM; doublet histone; giant virus; histone tail; non-eukaryotic nucleosome; nucleosome-like-particle; viral factory; viral nucleosome Substance Nomenclature: 0 (Fluorescent Dyes) ; 0 (Histones) ; 0 (Nucleosomes) Entry Date(s): Date Created: 20210723 Date Completed: 20220105 Latest Revision: 20220105 Update Code: 20231215 PubMed Central ID: PMC8357426

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Virus-encoded histone doublets are essential and form nucleosome-like structures.