Einzeltreffer — DigiBib

The homotetrameric DnaD protein is essential in low G+C content gram positive bacteria and is involved in replication initiation at oriC and re-start of collapsed replication forks. It interacts with the ubiquitously conserved bacterial master replication initiation protein DnaA at the oriC but structural and functional details of this interaction are lacking, thus contributing to our incomplete understanding of the molecular details that underpin replication initiation in bacteria. DnaD comprises N-terminal (DDBH1) and C-terminal (DDBH2) domains, with contradicting bacterial two-hybrid and yeast two-hybrid studies suggesting that either the former or the latter interact with DnaA, respectively. Using Nuclear Magnetic Resonance (NMR) we showed that both DDBH1 and DDBH2 interact with the N-terminal domain I of DnaA and studied the DDBH2 interaction in structural detail. We revealed two families of conformations for the DDBH2-DnaA domain I complex and showed that the DnaA-interaction patch of DnaD is distinct from the DNA-interaction patch, suggesting that DnaD can bind simultaneously DNA and DnaA. Using sensitive single-molecule FRET techniques we revealed that DnaD remodels DnaA-DNA filaments consistent with stretching and/or untwisting. Furthermore, the DNA binding activity of DnaD is redundant for this filament remodelling. This in turn suggests that DnaA and DnaD are working collaboratively in the oriC to locally melt the DNA duplex during replication initiation.

Titel:	DNA replication initiation in Bacillus subtilis: structural and functional characterization of the essential DnaA-DnaD interaction.
Autor/in / Beteiligte Person:	Martin, E ; Williams, HEL ; Pitoulias, M ; Stevens, D ; Winterhalter, C ; Craggs, TD ; Murray, H ; Searle, MS ; Soultanas, P
Zeitschrift:	Nucleic acids research, Jg. 47 (2019-02-28), Heft 4, S. 2101-2112
Veröffentlichung:	1992- : Oxford : Oxford University Press ; <i>Original Publication</i>: London, Information Retrieval ltd., 2019
Medientyp:	academicJournal
ISSN:	1362-4962 (electronic)
DOI:	10.1093/nar/gky1220
Schlagwort:	Bacillus subtilis genetics Bacterial Proteins chemistry DNA-Binding Proteins chemistry DnaB Helicases chemistry DnaB Helicases genetics Magnetic Resonance Spectroscopy Multiprotein Complexes chemistry Multiprotein Complexes genetics Origin Recognition Complex genetics Protein Binding genetics Protein Domains genetics Structure-Activity Relationship Bacterial Proteins genetics DNA Replication genetics DNA-Binding Proteins genetics Replication Origin genetics
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Nucleic Acids Res] 2019 Feb 28; Vol. 47 (4), pp. 2101-2112. MeSH Terms: Bacterial Proteins / genetics ; DNA Replication / genetics ; DNA-Binding Proteins / genetics ; Replication Origin / genetics ; Bacillus subtilis / genetics ; Bacterial Proteins / chemistry ; DNA-Binding Proteins / chemistry ; DnaB Helicases / chemistry ; DnaB Helicases / genetics ; Magnetic Resonance Spectroscopy ; Multiprotein Complexes / chemistry ; Multiprotein Complexes / genetics ; Origin Recognition Complex / genetics ; Protein Binding / genetics ; Protein Domains / genetics ; Structure-Activity Relationship References: J Biol Chem. 2010 Sep 3;285(36):28229-39. (PMID: 20595381) ; J Biol Chem. 1998 Dec 18;273(51):34255-62. (PMID: 9852089) ; Mol Microbiol. 2005 Feb;55(4):1138-50. (PMID: 15686560) ; Genes Genet Syst. 2008 Apr;83(2):111-25. (PMID: 18506095) ; Nature. 2011 Oct 02;478(7368):209-13. (PMID: 21964332) ; Genes (Basel). 2017 Jan 10;8(1):. (PMID: 28075389) ; Mol Cell. 2003 Apr;11(4):1009-20. (PMID: 12718886) ; Curr Opin Cell Biol. 2009 Dec;21(6):771-7. (PMID: 19767190) ; J Bacteriol. 2006 Aug;188(15):5487-93. (PMID: 16855238) ; J Mol Biol. 2005 Aug 5;351(1):66-75. (PMID: 16002087) ; J Bacteriol. 2012 Sep;194(18):5110-7. (PMID: 22821970) ; Nucleic Acids Res. 2017 Apr 20;45(7):3888-3905. (PMID: 28168278) ; Nucleic Acids Res. 2003 Apr 15;31(8):2077-86. (PMID: 12682358) ; Microbiology. 2007 Oct;153(Pt 10):3370-3379. (PMID: 17906136) ; J Mol Biol. 2008 Mar 28;377(3):706-14. (PMID: 18291414) ; J Biol Chem. 2009 Sep 11;284(37):25038-50. (PMID: 19632993) ; Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21115-20. (PMID: 19940251) ; J Mol Biol. 2008 Mar 7;376(5):1237-50. (PMID: 18206906) ; Nucleic Acids Res. 2012 Jan;40(2):739-50. (PMID: 21954439) ; Nat Commun. 2013;4:2131. (PMID: 23831915) ; Plasmid. 2009 Sep;62(2):71-82. (PMID: 19527752) ; J Bacteriol. 2001 Mar;183(6):2148-50. (PMID: 11222620) ; Genes Dev. 2017 Jun 1;31(11):1073-1088. (PMID: 28717046) ; J Bacteriol. 2012 Oct;194(19):5162-70. (PMID: 22797751) ; J Mol Biol. 2007 Mar 30;367(3):764-9. (PMID: 17289076) ; Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:1-16. (PMID: 23962882) ; EMBO J. 2012 Mar 21;31(6):1542-55. (PMID: 22286949) ; Biochem Cell Biol. 2010 Feb;88(1):49-62. (PMID: 20130679) ; Mol Microbiol. 2013 Oct;90(1):147-59. (PMID: 23909787) ; Nat Rev Microbiol. 2007 May;5(5):343-54. (PMID: 17435790) ; Nat Struct Mol Biol. 2006 Aug;13(8):676-83. (PMID: 16829961) ; Mol Microbiol. 2019 Jan;111(1):118-130. (PMID: 30285297) ; Mol Microbiol. 2014 Sep;93(5):975-91. (PMID: 25041308) ; Annu Rev Microbiol. 2006;60:351-75. (PMID: 16753031) ; Mol Microbiol. 2000 Sep;37(5):1270-9. (PMID: 10972842) ; Mol Genet Genomics. 2004 May;271(4):447-57. (PMID: 15042355) ; Proc Natl Acad Sci U S A. 2004 Jun 15;101(24):8936-41. (PMID: 15175430) ; Genes (Basel). 2017 May 10;8(5):. (PMID: 28489024) ; Mol Microbiol. 2006 May;60(4):917-24. (PMID: 16677303) ; Annu Rev Biochem. 2013;82:25-54. (PMID: 23746253) ; Nucleic Acids Res. 2010 Nov;38(20):6930-42. (PMID: 20587500) ; Mol Microbiol. 2012 Apr;84(1):6-16. (PMID: 22417087) ; Genes (Basel). 2017 Jan 11;8(1):. (PMID: 28085061) Grant Information: 204985/Z/16/Z United Kingdom WT_ Wellcome Trust Substance Nomenclature: 0 (Bacterial Proteins) ; 0 (DNA-Binding Proteins) ; 0 (DnaA protein, Bacteria) ; 0 (DnaD protein, Bacillus subtilis) ; 0 (Multiprotein Complexes) ; 0 (OriC chromosomal replication origin) ; 0 (Origin Recognition Complex) ; EC 3.6.4.12 (DnaB Helicases) Entry Date(s): Date Created: 20181212 Date Completed: 20190827 Latest Revision: 20230209 Update Code: 20231215 PubMed Central ID: PMC6393240

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

DNA replication initiation in Bacillus subtilis: structural and functional characterization of the essential DnaA-DnaD interaction.