IL (Interleukin)-10-STAT3-Galectin-3 Axis Is Essential for Osteopontin-Producing Reparative Macrophage Polarization After Myocardial Infarction.
In: Circulation, Jg. 138 (2018-10-30), Heft 18, S. 2021-2035
academicJournal
Zugriff:
Background: Both osteopontin (OPN) and galectin-3 have been implicated in phagocytic clearance of dead cells and reparative fibrosis during wound healing. CD206 + macrophages are involved in tissue repair through phagocytosis and fibrosis after myocardial infarction (MI). However, the relationship among OPN, galectin-3, and macrophage polarization in the context of MI remains unclear.
Methods: The time course of Spp1 (encoding OPN) expression in the heart after MI showed a strong activation of Spp1 on day 3 after MI. To identify where in the body and in which cells the transcriptional activity of Spp1 increased after MI, we analyzed EGFP (enhanced green fluorescent protein)- Spp1 knockin reporter mice on day 3 after MI.
Results: The transcriptional activity of Spp1 increased only in CD206 + macrophages in the infarct myocardium, and most of CD206 + macrophages have strong transcriptional activation of Spp1 after MI. The temporal expression pattern of Lgal3 (encoding galectin-3) in cardiac macrophages after MI was similar to that of Spp1, and OPN is almost exclusively produced by galectin-3 hi CD206 + macrophages. Although both interleukin (IL)-4 and IL-10 were reported to promote CD206 + macrophage-mediated cardiac repair after MI, IL-10- but not IL-4-stimulated CD11b + Ly6G - cells could differentiate into OPN-producing galectin-3 hi CD206 + macrophages and showed enhanced phagocytic ability. Inhibition of STAT3 tyrosine phosphorylation suppressed IL-10-induced expression of intracellular galectin-3 and transcriptional activation of Spp1. Knockdown of galectin-3 suppressed their ability to differentiate into OPN-producing cells, but not STAT3 activation. The tyrosine phosphorylation of STAT3 and the appearance rate of galectin-3 hi CD206 + cells on cardiac CD11b + Ly6G - cells in Spp1 knockout mice were the same as those in wild-type mice. Spp1 knockout mice showed vulnerability to developing post-MI left ventricular chamber dilatation and the terminal deoxynucleo-tidyltransferase 2'-Deoxyuridine-5'-triphosphate nick-end labeling (TUNEL)-positive cells in the infarcted myocardium after MI remained higher in number in Spp1 knockout mice than in wild-type mice.
Conclusions: OPN is almost exclusively produced by galectin-3 hi CD206 + macrophages, which specifically appear in the infarct myocardium after MI. The IL-10-STAT3-galectin-3 axis is essential for OPN-producing reparative macrophage polarization after myocardial infarction, and these macrophages contribute to tissue repair by promoting fibrosis and clearance of apoptotic cells. These results suggest that galectin-3 may contribute to reparative fibrosis in the infarct myocardium by controlling OPN levels.
Titel: |
IL (Interleukin)-10-STAT3-Galectin-3 Axis Is Essential for Osteopontin-Producing Reparative Macrophage Polarization After Myocardial Infarction.
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Autor/in / Beteiligte Person: | Shirakawa, K ; Endo, J ; Kataoka, M ; Katsumata, Y ; Yoshida, N ; Yamamoto, T ; Isobe, S ; Moriyama, H ; Goto, S ; Kitakata, H ; Hiraide, T ; Fukuda, K ; Sano, M |
Zeitschrift: | Circulation, Jg. 138 (2018-10-30), Heft 18, S. 2021-2035 |
Veröffentlichung: | Hagerstown, MD : Lippincott Williams & Wilkins ; <i>Original Publication</i>: [Dallas, Tex., etc., American Heart Association, etc.], 2018 |
Medientyp: | academicJournal |
ISSN: | 1524-4539 (electronic) |
DOI: | 10.1161/CIRCULATIONAHA.118.035047 |
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