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Background: α-glucosidase is a therapeutic target for diabetes mellitus (DM) and α-glucosidase inhibitors play a vital role in the treatments for the disease. Furthermore, xanthine oxidase (XO) is a key enzyme that catalyzes hypoxanthine and xanthine to uric acid which at high levels can lead to hyperuricemia which is an important cause of gout. Pancreatic lipase (PL) secreted into the duodenum plays a key role in the digestion and absorption of fats. For its importance in lipid digestion, PL represents an attractive target for obesity prevention.

Methods: The flowers essential oil of Rhaponticum acaule (L) DC (R. acaule) was characterized using gas chromatography-mass spectrometry (GC-MS). The antioxidant activities of R. acaule essential oil (RaEO) were also determined using 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), reducing power, phosphomolybdenum, and DNA nicking assays. The inhibitory power of RaEO against α-glucosidase, xanthine oxidase and pancreatic lipase was evaluated. Enzyme kinetic studies using Michaelis-Menten and the derived Lineweaver-Burk (LB) plots were performed to understand the possible mechanism of inhibition exercised by the components of this essential oil.

Results: The result revealed the presence of 26 compounds (97.4%). The main constituents include germacrene D (49.2%), methyl eugenol (8.3%), (E)-β-ionone (6.2%), β-caryophyllene (5.7%), (E,E)-α-farnesene (4.2%), bicyclogermacrene (4.1%) and (Z)-α-bisabolene (3.7%). The kinetic inhibition study showed that the essential oil demonstrated a strong α-glucosidase inhibiton and it was a mixed inhibitor. On the other hand, our results evidenced that this oil exhibited important xanthine oxidase inhibitory effect, behaving as a non-competitive inhibitor. The essential oil inhibited the turkey pancreatic lipase, with maximum inhibition of 80% achieved at 2 mg/mL. Furthermore, the inhibition of turkey pancreatic lipase by RaEO was an irreversible one.

Conclusion: The results revealed that the RaEO is a new promising potential source of antioxidant compounds, endowed with good practical applications for human health.

Titel:	Rhaponticum acaule (L) DC essential oil: chemical composition, in vitro antioxidant and enzyme inhibition properties.
Autor/in / Beteiligte Person:	Mosbah, H ; Chahdoura, H ; Kammoun, J ; Hlila, MB ; Louati, H ; Hammami, S ; Flamini, G ; Achour, L ; Selmi, B
Zeitschrift:	BMC complementary and alternative medicine, Jg. 18 (2018-03-05), Heft 1, S. 79
Veröffentlichung:	London : BioMed Central, [2001-2019], 2018
Medientyp:	academicJournal
ISSN:	1472-6882 (electronic)
DOI:	10.1186/s12906-018-2145-5
Schlagwort:	Antioxidants isolation & purification Aspergillus niger enzymology Enzyme Inhibitors isolation & purification Fungal Proteins antagonists & inhibitors Fungal Proteins chemistry Gas Chromatography-Mass Spectrometry Glycoside Hydrolase Inhibitors isolation & purification Kinetics Oils, Volatile isolation & purification Xanthine Oxidase antagonists & inhibitors Xanthine Oxidase chemistry alpha-Glucosidases chemistry Antioxidants chemistry Enzyme Inhibitors chemistry Glycoside Hydrolase Inhibitors chemistry Leuzea chemistry Oils, Volatile chemistry
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [BMC Complement Altern Med] 2018 Mar 05; Vol. 18 (1), pp. 79. <i>Date of Electronic Publication: </i>2018 Mar 05. MeSH Terms: Antioxidants / chemistry ; Enzyme Inhibitors / chemistry ; Glycoside Hydrolase Inhibitors / chemistry ; Leuzea / chemistry ; Oils, Volatile / *chemistry ; Antioxidants / isolation & purification ; Aspergillus niger / enzymology ; Enzyme Inhibitors / isolation & purification ; Fungal Proteins / antagonists & inhibitors ; Fungal Proteins / chemistry ; Gas Chromatography-Mass Spectrometry ; Glycoside Hydrolase Inhibitors / isolation & purification ; Kinetics ; Oils, Volatile / isolation & purification ; Xanthine Oxidase / antagonists & inhibitors ; Xanthine Oxidase / chemistry ; alpha-Glucosidases / chemistry References: Nat Prod Commun. 2016 Apr;11(4):535-8. (PMID: 27396212) ; Evid Based Complement Alternat Med. 2014;2014:467363. (PMID: 25050126) ; Phytochemistry. 2009 Feb;70(3):414-8. (PMID: 19195668) ; Biochim Biophys Acta. 1991 Oct 1;1085(3):322-8. (PMID: 1911866) ; J Ethnopharmacol. 2000 Nov;73(1-2):199-207. (PMID: 11025157) ; Chirality. 2005 Jan;17(1):57-62. (PMID: 15549712) ; J Biol Chem. 1985 Feb 25;260(4):2268-73. (PMID: 4038705) ; Biochimie. 1975;57(10):1117-22. (PMID: 1222120) ; J Nat Med. 2009 Jul;63(3):355-9. (PMID: 19387769) ; Pharmacogn Rev. 2011 Jan;5(9):82-95. (PMID: 22096322) ; J Oleo Sci. 2009;58(8):443-6. (PMID: 19584571) ; Biomed Chromatogr. 2013 Feb;27(2):148-55. (PMID: 22674728) ; J Food Sci. 2014 Jul;79(7):R1231-49. (PMID: 24888440) ; Food Chem Toxicol. 2008 Feb;46(2):446-75. (PMID: 17996351) ; J Pharm Sci. 1993 Jun;82(6):660-4. (PMID: 8331544) ; Oxid Med Cell Longev. 2016;2016:8505384. (PMID: 28074117) ; Food Chem Toxicol. 2013 Jun;56:352-62. (PMID: 23459148) ; Nat Rev Drug Discov. 2002 Sep;1(9):696-709. (PMID: 12209150) ; J Agric Food Chem. 2002 Oct 23;50(22):6490-6. (PMID: 12381138) ; Food Chem Toxicol. 2013 Sep;59:586-94. (PMID: 23831310) ; Planta Med. 1984 Jun;50(3):205-9. (PMID: 6484028) ; Biochemistry. 1986 Apr 8;25(7):1733-8. (PMID: 3707907) ; Food Sci Biotechnol. 2016 Jun 30;25(3):687-693. (PMID: 30263324) ; Phytochemistry. 2009 May;70(7):842-55. (PMID: 19457517) ; Anal Biochem. 1999 May 1;269(2):337-41. (PMID: 10222007) ; Free Radic Biol Med. 1999 May;26(9-10):1231-7. (PMID: 10381194) ; Microbiol Res. 2008;163(1):87-95. (PMID: 17482441) ; Food Chem. 2013 Nov 15;141(2):1412-5. (PMID: 23790932) Contributed Indexing: Keywords: Antioxidant activity; Chemical composition; Pancreatic lipase inhibition; Rhaponticum acaule essential oil; Xanthine oxidase; α-glucosidase Substance Nomenclature: 0 (Antioxidants) ; 0 (Enzyme Inhibitors) ; 0 (Fungal Proteins) ; 0 (Glycoside Hydrolase Inhibitors) ; 0 (Oils, Volatile) ; EC 1.17.3.2 (Xanthine Oxidase) ; EC 3.2.1.20 (alpha-Glucosidases) Entry Date(s): Date Created: 20180307 Date Completed: 20180412 Latest Revision: 20240313 Update Code: 20240313 PubMed Central ID: PMC5838999

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Rhaponticum acaule (L) DC essential oil: chemical composition, in vitro antioxidant and enzyme inhibition properties.