[Activity of essential oil extracted from Artemisia argyi in inducing apoptosis of Candida albicans].
In: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, Jg. 42 (2017-09-01), Heft 18, S. 3572-3577
academicJournal
Zugriff:
To explore the activity of essential oil extracted from Artemisia argyi (AAEO) in inducing the apoptosis of Candida albicans SC5314. The effect of AAEO on reactive oxygen species(ROS) and mitochondria membrane potential(MMP) of C. albicans SC5314 was detected by flow cytometry. Phosphatidylserine externalization was observed under fluorescence microscopic with Annexin-V/PI staining at the early stage of apoptosis in C. albicans. Metacaspase activity was observed under fluorescence microscopic with FITC-VAD-FMK staining at the early stage of apoptosis in C. albicans. C. albicans morphology was observed by DAPI nuclear staining and fluorescence microscopy. After intervention with 0.5 mL•L⁻¹ AAEO, apoptosis of C. albicans significantly increased, metacaspase activity increased, nuclear pyknosis and fragmentation, and intracellular ROS were significantly increased, and mitochondrial membrane potential decreased significantly. The certain concentrations of AAEO could induce the apoptosis of C. albicans.
Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.
(Copyright© by the Chinese Pharmaceutical Association.)
Titel: |
[Activity of essential oil extracted from Artemisia argyi in inducing apoptosis of Candida albicans].
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Autor/in / Beteiligte Person: | Shi, GX ; Wang, TM ; Wu, SB ; Wang, YX ; Shao, J ; Zhou, MQ ; Wang, CZ |
Zeitschrift: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, Jg. 42 (2017-09-01), Heft 18, S. 3572-3577 |
Veröffentlichung: | Beijing : Zhongguo yao xue hui : Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo ; <i>Original Publication</i>: [Beijing] : Zhongguo yao xue hui : [Zhongguo Zhong yi yan jiu yuan Zhong yao yan jiu suo, 1989-, 2017 |
Medientyp: | academicJournal |
ISSN: | 1001-5302 (print) |
DOI: | 10.19540/j.cnki.cjcmm.2017.0136 |
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