Einzeltreffer — DigiBib

The spliceosome is not a single macromolecular machine. Rather it is a collection of dynamic heterogeneous subcomplexes that rapidly interconvert throughout the course of a typical splicing cycle. Because of this, for many years the only high resolution structures of the spliceosome available were of smaller, isolated protein or RNA components. Consequently much of our current understanding of the spliceosome derives from biochemical and genetic techniques. Now with the publication of multiple, high resolution structures of the spliceosome, some question the relevance of traditional biochemical and genetic techniques to the splicing field. We argue such techniques are not only relevant, but vital for an in depth mechanistic understanding of pre-mRNA splicing.

Titel:	Genetics and biochemistry remain essential in the structural era of the spliceosome.
Autor/in / Beteiligte Person:	Mayerle, M ; Guthrie, C
Zeitschrift:	Methods (San Diego, Calif.), Jg. 125 (2017-08-01), S. 3-9
Veröffentlichung:	Duluth, MN : Academic Press ; <i>Original Publication</i>: San Diego : Academic Press, c1990-, 2017
Medientyp:	academicJournal
ISSN:	1095-9130 (electronic)
DOI:	10.1016/j.ymeth.2017.01.006
Schlagwort:	Eukaryota genetics Eukaryota metabolism Humans Introns genetics RNA Precursors genetics RNA Precursors metabolism Spliceosomes chemistry Spliceosomes genetics Biochemistry methods Molecular Biology methods Molecular Imaging methods RNA Splicing Spliceosomes ultrastructure
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Genetics and biochemistry remain essential in the structural era of the spliceosome.