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Escherichia coli single-stranded DNA binding protein (SSB) is an essential homotetramer that binds ssDNA and recruits multiple proteins to their sites of action during genomic maintenance. Each SSB subunit contains an N-terminal globular oligonucleotide/oligosaccharide binding fold (OB-fold) and an intrinsically disordered C-terminal domain. SSB binds ssDNA in multiple modes in vitro, including the fully wrapped (SSB)65 and (SSB)56 modes, in which ssDNA contacts all four OB-folds, and the highly cooperative (SSB)35 mode, in which ssDNA contacts an average of only two OB-folds. These modes can both be populated under physiological conditions. While these different modes might be used for different functions, this has been difficult to assess. Here we used a dimeric SSB construct with two covalently linked OB-folds to disable ssDNA binding in two of the four OB-folds thus preventing formation of fully wrapped DNA complexes in vitro, although they retain a wild-type-like, salt-dependent shift in cooperative binding to ssDNA. These variants complement wild-type SSB in vivo indicating that a fully wrapped mode is not essential for function. These results do not preclude a normal function for a fully wrapped mode, but do indicate that E. coli tolerates some flexibility with regards to its SSB binding modes.

Titel:	Is a fully wrapped SSB-DNA complex essential for Escherichia coli survival?
Autor/in / Beteiligte Person:	Waldman, VM ; Weiland, E ; Kozlov, AG ; Lohman, TM
Zeitschrift:	Nucleic acids research, Jg. 44 (2016-05-19), Heft 9, S. 4317-29
Veröffentlichung:	1992- : Oxford : Oxford University Press ; <i>Original Publication</i>: London, Information Retrieval ltd., 2016
Medientyp:	academicJournal
ISSN:	1362-4962 (electronic)
DOI:	10.1093/nar/gkw262
Schlagwort:	Binding Sites DNA Damage DNA, Bacterial chemistry DNA, Bacterial genetics DNA, Single-Stranded chemistry DNA-Binding Proteins chemistry Escherichia coli Proteins chemistry Microbial Viability Protein Binding DNA-Binding Proteins physiology Escherichia coli physiology Escherichia coli Proteins physiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Nucleic Acids Res] 2016 May 19; Vol. 44 (9), pp. 4317-29. <i>Date of Electronic Publication: </i>2016 Apr 15. MeSH Terms: DNA-Binding Proteins / physiology ; Escherichia coli / physiology ; Escherichia coli Proteins / *physiology ; Binding Sites ; DNA Damage ; DNA, Bacterial / chemistry ; DNA, Bacterial / genetics ; DNA, Single-Stranded / chemistry ; DNA-Binding Proteins / chemistry ; Escherichia coli Proteins / chemistry ; Microbial Viability ; Protein Binding References: J Mol Biol. 2013 Nov 29;425(23):4802-19. (PMID: 24021816) ; Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1373-8. (PMID: 24379377) ; Biochemistry. 2014 Apr 1;53(12):1925-34. (PMID: 24606314) ; J Mol Biol. 2014 Jun 26;426(13):2413-21. (PMID: 24792418) ; J Mol Biol. 2014 Sep 23;426(19):3246-61. (PMID: 25058683) ; J Mol Biol. 2015 Feb 27;427(4):763-74. (PMID: 25562210) ; Elife. 2015;4. doi: 10.7554/eLife.08193. 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(PMID: 22976177) ; J Biol Chem. 2013 Jun 14;288(24):17569-78. (PMID: 23629733) Grant Information: R01 GM030498 United States GM NIGMS NIH HHS Substance Nomenclature: 0 (DNA, Bacterial) ; 0 (DNA, Single-Stranded) ; 0 (DNA-Binding Proteins) ; 0 (Escherichia coli Proteins) ; 0 (SSB protein, E coli) Entry Date(s): Date Created: 20160417 Date Completed: 20170704 Latest Revision: 20201209 Update Code: 20240513 PubMed Central ID: PMC4872115

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Is a fully wrapped SSB-DNA complex essential for Escherichia coli survival?