Essential role of flotillin-1 palmitoylation in the intracellular localization and signaling function of IGF-1 receptor.
In: Journal of cell science, Jg. 128 (2015-06-01), Heft 11, S. 2179-90
academicJournal
Zugriff:
Here, we explored flotillin-1-mediated regulation of insulin-like growth factor-1 (IGF-1) signaling. Flotillin-1-deficient cells exhibited a reduction in the activation of IGF-1 receptor (IGF-1R), ERK1/2 and Akt pathways, and the transcriptional activation of Elk-1 and the proliferation in response to IGF-1 were reduced in these cells. We found that IGF-1-independent flotillin-1 palmitoylation at Cys34 in the endoplasmic reticulum (ER) was required for the ER exit and the plasma membrane localization of flotillin-1 and IGF-1R. IGF-1-dependent depalmitoylation and repalmitoylation of flotillin-1 sustained tyrosine kinase activation of the plasma-membrane-targeted IGF-1R. Dysfunction and blocking the turnover of flotillin-1 palmitoylation abrogated cancer cell proliferation after IGF-1R signaling activation. Our data show that flotillin-1 palmitoylation is a new mechanism by which the intracellular localization and activation of IGF-1R are controlled.
(© 2015. Published by The Company of Biologists Ltd.)
Titel: |
Essential role of flotillin-1 palmitoylation in the intracellular localization and signaling function of IGF-1 receptor.
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Autor/in / Beteiligte Person: | Jang, D ; Kwon, H ; Jeong, K ; Lee, J ; Pak, Y |
Zeitschrift: | Journal of cell science, Jg. 128 (2015-06-01), Heft 11, S. 2179-90 |
Veröffentlichung: | Cambridge : Company of Biologists ; <i>Original Publication</i>: London., 2015 |
Medientyp: | academicJournal |
ISSN: | 1477-9137 (electronic) |
DOI: | 10.1242/jcs.169409 |
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