Serine 192 in the tiny RS repeat of the adenoviral L4-33K splicing enhancer protein is essential for function and reorganization of the protein to the periphery of viral replication centers.

Östberg, S ; Törmänen Persson, H ; et al.

In: Virology, Jg. 433 (2012-11-25), Heft 2, S. 273-81

academicJournal

The adenovirus L4-33K protein is a key regulator involved in the temporal shift from early to late pattern of mRNA expression from the adenovirus major late transcription unit. L4-33K is a virus-encoded alternative splicing factor, which enhances processing of 3' splice sites with a weak sequence context. Here we show that L4-33K expressed from a plasmid is localized at the nuclear margin of uninfected cells. During an infection L4-33K is relocalized to the periphery of E2A-72K containing viral replication centers. We also show that serine 192 in the tiny RS repeat of the conserved carboxy-terminus of L4-33K, which is critical for the splicing enhancer function of L4-33K, is necessary for the nuclear localization and redistribution of the protein to viral replication sites. Collectively, our results show a good correlation between the activity of L4-33K as a splicing enhancer protein and its localization to the periphery of viral replication centers.

Titel:	Serine 192 in the tiny RS repeat of the adenoviral L4-33K splicing enhancer protein is essential for function and reorganization of the protein to the periphery of viral replication centers.
Autor/in / Beteiligte Person:	Östberg, S ; Törmänen Persson, H ; Akusjärvi, G
Zeitschrift:	Virology, Jg. 433 (2012-11-25), Heft 2, S. 273-81
Veröffentlichung:	New York, Academic Press., 2012
Medientyp:	academicJournal
ISSN:	1096-0341 (electronic)
DOI:	10.1016/j.virol.2012.08.021
Schlagwort:	Alternative Splicing Amino Acid Motifs Amino Acid Sequence Cell Nucleus metabolism Cell Nucleus virology Enhancer Elements, Genetic Gene Expression Regulation, Viral HEK293 Cells Humans Molecular Sequence Data Mutant Proteins chemistry Mutant Proteins genetics Mutant Proteins metabolism Protein Transport RNA, Messenger genetics RNA, Messenger metabolism RNA, Viral genetics RNA, Viral metabolism Repetitive Sequences, Amino Acid Serine chemistry Viral Nonstructural Proteins chemistry Viral Nonstructural Proteins genetics Viral Nonstructural Proteins metabolism Viral Proteins chemistry Virus Replication Adenoviruses, Human genetics Adenoviruses, Human metabolism Viral Proteins genetics Viral Proteins metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [Virology] 2012 Nov 25; Vol. 433 (2), pp. 273-81. <i>Date of Electronic Publication: </i>2012 Sep 01. MeSH Terms: Adenoviruses, Human / genetics ; Adenoviruses, Human / metabolism ; Viral Proteins / genetics ; Viral Proteins / metabolism ; Alternative Splicing ; Amino Acid Motifs ; Amino Acid Sequence ; Cell Nucleus / metabolism ; Cell Nucleus / virology ; Enhancer Elements, Genetic ; Gene Expression Regulation, Viral ; HEK293 Cells ; Humans ; Molecular Sequence Data ; Mutant Proteins / chemistry ; Mutant Proteins / genetics ; Mutant Proteins / metabolism ; Protein Transport ; RNA, Messenger / genetics ; RNA, Messenger / metabolism ; RNA, Viral / genetics ; RNA, Viral / metabolism ; Repetitive Sequences, Amino Acid ; Serine / chemistry ; Viral Nonstructural Proteins / chemistry ; Viral Nonstructural Proteins / genetics ; Viral Nonstructural Proteins / metabolism ; Viral Proteins / chemistry ; Virus Replication Substance Nomenclature: 0 (L4-22K protein, adenovirus) ; 0 (Mutant Proteins) ; 0 (RNA, Messenger) ; 0 (RNA, Viral) ; 0 (Viral Nonstructural Proteins) ; 0 (Viral Proteins) ; 452VLY9402 (Serine) Entry Date(s): Date Created: 20120905 Date Completed: 20130110 Latest Revision: 20131121 Update Code: 20231215

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