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Myosin X (Myo10) with pleckstrin homology (PH) domains is a motor protein acting in filopodium initiation and extension. However, its potential role has not been fully understood, especially in neuronal development. In the present study the preferential accumulation of Myo10 in axon tips has been revealed in primary culture of hippocampal neurons with the aid of immunofluorescence from anti-Myo10 antibody in combination with anti-Tuj1 antibody as specific marker. Knocking down Myo10 gene transcription impaired outgrowth of axon with loss of Tau-1-positive phenotype. Interestingly, inhibition of actin polymerization by cytochalasin D rescued the defect of axon outgrowth. Furthermore, ectopic expression of Myo10 with enhanced green fluorescence protein (EGFP) labeled Myo10 mutants induced multiple axon-like neurites in a motor-independent way. Mechanism studies demonstrated that the recruitment of Myo10 through its PH domain to phosphatidylinositol (3,4,5)-trisphosphate (PtdIns (3,4,5) P3) was essential for axon formation. In addition, in vivo studies confirmed that Myo10 was required for neuronal morphological transition during radial neuronal migration in the developmental neocortex.

Titel:	PtdIns (3,4,5) P3 recruitment of Myo10 is essential for axon development.
Autor/in / Beteiligte Person:	Yu, H ; Wang, N ; Ju, X ; Yang, Y ; Sun, D ; Lai, M ; Cui, L ; Sheikh, MA ; Zhang, J ; Wang, X ; Zhu, X
Link:	Volltext (PDF)
Zeitschrift:	PloS one, Jg. 7 (2012), Heft 5, S. e36988
Veröffentlichung:	San Francisco, CA : Public Library of Science, 2012
Medientyp:	academicJournal
ISSN:	1932-6203 (electronic)
DOI:	10.1371/journal.pone.0036988
Schlagwort:	Actins genetics Actins metabolism Animals Antibodies, Monoclonal genetics Antibodies, Monoclonal metabolism Cell Movement drug effects Cells, Cultured Cytochalasin D pharmacology Gene Knockdown Techniques Hippocampus cytology Hippocampus metabolism Mice Mutation Myosins genetics Neocortex cytology Neocortex metabolism Nerve Tissue Proteins genetics Nucleic Acid Synthesis Inhibitors pharmacology Axons metabolism Cell Movement physiology Hippocampus embryology Myosins biosynthesis Neocortex embryology Nerve Tissue Proteins biosynthesis Phosphatidylinositol Phosphates metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [PLoS One] 2012; Vol. 7 (5), pp. e36988. <i>Date of Electronic Publication: </i>2012 May 10. MeSH Terms: Axons / metabolism ; Cell Movement / physiology ; Hippocampus / embryology ; Myosins / biosynthesis ; Neocortex / embryology ; Nerve Tissue Proteins / biosynthesis ; Phosphatidylinositol Phosphates / *metabolism ; Actins / genetics ; Actins / metabolism ; Animals ; Antibodies, Monoclonal / genetics ; Antibodies, Monoclonal / metabolism ; Cell Movement / drug effects ; Cells, Cultured ; Cytochalasin D / pharmacology ; Gene Knockdown Techniques ; Hippocampus / cytology ; Hippocampus / metabolism ; Mice ; Mutation ; Myosins / genetics ; Neocortex / cytology ; Neocortex / metabolism ; Nerve Tissue Proteins / genetics ; Nucleic Acid Synthesis Inhibitors / pharmacology References: FEBS Lett. 2008 Jul 9;582(16):2377-81. (PMID: 18570893) ; Mol Cell Biol. 2010 Apr;30(7):1703-17. (PMID: 20123970) ; J Cell Biol. 2008 Jul 14;182(1):77-88. (PMID: 18606852) ; Neuron. 2007 Nov 8;56(3):441-55. (PMID: 17988629) ; J Cell Sci. 2010 Oct 15;123(Pt 20):3525-34. (PMID: 20930142) ; Curr Opin Neurobiol. 2008 Oct;18(5):479-87. (PMID: 18929658) ; Nat Neurosci. 2004 Sep;7(9):923-9. (PMID: 15286792) ; J Cell Sci. 2007 Jan 15;120(Pt 2):279-88. (PMID: 17200137) ; Dev Neurobiol. 2009 Apr;69(5):279-98. (PMID: 19224562) ; Mol Biol Cell. 2008 Apr;19(4):1561-74. (PMID: 18256280) ; J Biol Chem. 2004 Mar 26;279(13):12804-11. (PMID: 14676191) ; Nat Cell Biol. 2007 Jul;9(7):743-54. (PMID: 17558396) ; J Cell Sci. 2008 Mar 15;121(Pt 6):796-803. (PMID: 18285448) ; Biochem Biophys Res Commun. 2006 Feb 3;340(1):62-8. (PMID: 16343426) ; Nat Cell Biol. 2007 Dec;9(12):1347-59. (PMID: 18026093) ; Science. 1999 Mar 19;283(5409):1931-4. (PMID: 10082468) ; Annu Rev Neurosci. 2009;32:347-81. (PMID: 19400726) ; Biochem Biophys Res Commun. 2004 Jun 18;319(1):214-20. (PMID: 15158464) ; Nat Cell Biol. 2002 Jul;4(7):469-77. (PMID: 12055636) ; Dev Neurobiol. 2009 Oct;69(12):761-79. (PMID: 19513994) ; Cell. 2005 Jan 14;120(1):123-35. (PMID: 15652487) ; J Cell Sci. 2006 Jan 1;119(Pt 1):184-94. (PMID: 16371656) ; J Neurochem. 2004 Apr;89(1):109-18. (PMID: 15030394) ; Proc Natl Acad Sci U S A. 2006 Aug 15;103(33):12411-6. (PMID: 16894163) ; J Cell Biol. 2007 Dec 31;179(7):1569-82. (PMID: 18158328) ; Dev Neurobiol. 2011 Jun;71(6):430-44. (PMID: 21557499) ; Nat Cell Biol. 2007 Feb;9(2):184-92. (PMID: 17237772) ; Nat Struct Mol Biol. 2011 Jun 12;18(7):783-8. (PMID: 21666676) ; Nat Cell Biol. 2004 Jun;6(6):523-31. (PMID: 15156152) ; J Biol Chem. 2008 Jul 18;283(29):20454-72. (PMID: 18448434) ; J Neurosci. 2007 Nov 28;27(48):13117-29. (PMID: 18045906) ; Prog Neurobiol. 2011 Jul;94(2):91-101. (PMID: 21527310) ; J Neurosci. 2011 Jan 26;31(4):1528-38. (PMID: 21273437) ; J Neurosci. 1988 Apr;8(4):1454-68. (PMID: 3282038) ; Trends Cell Biol. 2005 Oct;15(10):533-9. (PMID: 16140532) Substance Nomenclature: 0 (Actins) ; 0 (Antibodies, Monoclonal) ; 0 (Myo10 protein, mouse) ; 0 (Nerve Tissue Proteins) ; 0 (Nucleic Acid Synthesis Inhibitors) ; 0 (Phosphatidylinositol Phosphates) ; 0 (phosphatidylinositol 3,4,5-triphosphate) ; 0 (tau-1 monoclonal antibody) ; 22144-77-0 (Cytochalasin D) ; EC 3.6.4.1 (Myosins) Entry Date(s): Date Created: 20120517 Date Completed: 20120919 Latest Revision: 20240317 Update Code: 20240317 PubMed Central ID: PMC3349655

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PtdIns (3,4,5) P3 recruitment of Myo10 is essential for axon development.