Einzeltreffer — DigiBib

Tel1/ATM, a conserved phosphatidylinositol 3-kinase-related kinase (PIKK), acts in the response to DNA damage and regulates telomere maintenance. PIKK family members share an extended N-terminal region of low sequence homology. Sequence alignment of the N terminus of Tel1/ATM orthologs revealed a conserved, novel motif we term TAN (for Tel1/ATM N-terminal motif). Point mutations in conserved residues of the TAN motif resulted in telomere shortening, and its deletion caused the same short telomere phenotype as complete deletion of Tel1 did. Overexpressing Tel1 TAN mutants did not rescue telomere shortening. The TAN motif was also essential for the function of Tel1 in the response to DNA damage, as TAN-deleted Tel1 was indistinguishable from the complete lack of Tel1 in causing reduced viability and signaling through Rad53 upon DNA damage. Strikingly, TAN deletion reduced recruitment of Tel1 to a double-strand DNA break. Together, these results define a conserved sequence motif within an otherwise poorly defined region of the Tel1/ATM kinase family proteins that is essential for normal Tel1 function in Saccharomyces cerevisiae.

Titel:	A novel Tel1/ATM N-terminal motif, TAN, is essential for telomere length maintenance and a DNA damage response.
Autor/in / Beteiligte Person:	Seidel, JJ ; Anderson, CM ; Blackburn, EH
Zeitschrift:	Molecular and cellular biology, Jg. 28 (2008-09-01), Heft 18, S. 5736-46
Veröffentlichung:	2023- : [Philadelphia] : Taylor & Francis ; <i>Original Publication</i>: [Washington, D.C.] : American Society for Microbiology, [c1981-, 2008
Medientyp:	academicJournal
ISSN:	1098-5549 (electronic)
DOI:	10.1128/MCB.00326-08
Schlagwort:	Animals Ataxia Telangiectasia Mutated Proteins Cell Cycle Proteins genetics Cell Nucleus metabolism DNA Repair DNA Repair Enzymes genetics DNA Repair Enzymes metabolism DNA-Binding Proteins genetics Humans Molecular Sequence Data Protein Serine-Threonine Kinases genetics Saccharomyces cerevisiae Proteins genetics Sequence Alignment Sequence Homology, Amino Acid Telomere genetics Tumor Suppressor Proteins genetics Amino Acid Sequence Cell Cycle Proteins metabolism DNA Damage DNA-Binding Proteins metabolism Intracellular Signaling Peptides and Proteins genetics Intracellular Signaling Peptides and Proteins metabolism Protein Serine-Threonine Kinases metabolism Saccharomyces cerevisiae genetics Saccharomyces cerevisiae metabolism Saccharomyces cerevisiae Proteins metabolism Telomere metabolism Tumor Suppressor Proteins metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Language: English [Mol Cell Biol] 2008 Sep; Vol. 28 (18), pp. 5736-46. <i>Date of Electronic Publication: </i>2008 Jul 14. MeSH Terms: Amino Acid Sequence* ; DNA Damage* ; Intracellular Signaling Peptides and Proteins* / genetics ; Intracellular Signaling Peptides and Proteins* / metabolism ; Saccharomyces cerevisiae* / genetics ; Saccharomyces cerevisiae* / metabolism ; Cell Cycle Proteins / metabolism ; DNA-Binding Proteins / metabolism ; Protein Serine-Threonine Kinases / metabolism ; Saccharomyces cerevisiae Proteins / metabolism ; Telomere / metabolism ; Tumor Suppressor Proteins / metabolism ; Animals ; Ataxia Telangiectasia Mutated Proteins ; Cell Cycle Proteins / genetics ; Cell Nucleus / metabolism ; DNA Repair ; DNA Repair Enzymes / genetics ; DNA Repair Enzymes / metabolism ; DNA-Binding Proteins / genetics ; Humans ; Molecular Sequence Data ; Protein Serine-Threonine Kinases / genetics ; Saccharomyces cerevisiae Proteins / genetics ; Sequence Alignment ; Sequence Homology, Amino Acid ; Telomere / genetics ; Tumor Suppressor Proteins / genetics References: Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13749-54. (PMID: 11095737) ; Mol Cell Biol. 2004 May;24(9):4019-31. (PMID: 15082794) ; J Biol Chem. 2005 Jul 29;280(30):27587-94. (PMID: 15929992) ; Nucleic Acids Res. 2000 Apr 15;28(8):1692-9. (PMID: 10734187) ; Science. 1995 Jun 23;268(5218):1749-53. (PMID: 7792600) ; Nat Genet. 1995 Oct;11(2):115-6. (PMID: 7550332) ; Genes Dev. 2007 Jul 15;21(14):1726-30. (PMID: 17639079) ; Cell. 2004 Sep 17;118(6):699-713. (PMID: 15369670) ; Proc Natl Acad Sci U S A. 1986 Mar;83(5):1398-402. (PMID: 3513174) ; Methods Enzymol. 2002;351:394-408. (PMID: 12073359) ; Mol Cell. 2006 Nov 17;24(4):603-10. (PMID: 17188035) ; Genes Dev. 2003 Aug 15;17(16):1957-62. (PMID: 12923051) ; Curr Biol. 2001 Aug 21;11(16):1240-50. (PMID: 11525738) ; Nat Genet. 1996 Jul;13(3):350-3. (PMID: 8673136) ; Mol Cell Biol. 2005 Jul;25(13):5363-79. (PMID: 15964794) ; Mol Cell. 2007 Sep 7;27(5):851-8. (PMID: 17803948) ; Nature. 2005 Mar 31;434(7033):605-11. (PMID: 15758953) ; Mol Cell. 2007 Aug 17;27(4):550-61. (PMID: 17656141) ; J Biol Chem. 2005 Apr 15;280(15):15158-64. (PMID: 15713674) ; Yeast. 1998 Jul;14(10):953-61. (PMID: 9717241) ; Nature. 2003 May 15;423(6937):241-54. (PMID: 12748633) ; Genes Dev. 2008 Apr 1;22(7):854-9. (PMID: 18334620) ; Trends Biochem Sci. 2000 May;25(5):225-7. (PMID: 10782091) ; EMBO Rep. 2005 Aug;6(8):723-8. (PMID: 16065066) ; Mol Cell. 2001 Jun;7(6):1255-66. (PMID: 11430828) ; Cell. 2003 Jan 24;112(2):151-5. (PMID: 12553904) ; Mol Cell Biol. 1999 Sep;19(9):6065-75. (PMID: 10454554) ; EMBO J. 2006 Feb 22;25(4):846-56. (PMID: 16467854) ; Mol Cell Biol. 1999 Oct;19(10):6963-71. (PMID: 10490633) ; J Biol Chem. 2002 Aug 23;277(34):30515-23. (PMID: 12034743) ; Curr Biol. 2001 Sep 4;11(17):1328-35. (PMID: 11553325) ; Yeast. 1995 Oct;11(13):1265-74. (PMID: 8553697) ; Science. 2003 Jul 4;301(5629):71-6. (PMID: 12775844) ; Mol Cell Biol. 2001 Mar;21(5):1710-8. (PMID: 11238908) ; Cell. 1995 Sep 8;82(5):823-9. (PMID: 7671310) ; Science. 2002 Aug 9;297(5583):1023-6. (PMID: 12169735) ; Oncogene. 2001 Aug 23;20(37):5100-10. (PMID: 11526498) ; Nat Genet. 1998 Dec;20(4):398-400. (PMID: 9843217) ; J Struct Biol. 2004 Mar;145(3):295-306. (PMID: 14960380) ; Cancer Res. 2000 Jun 15;60(12):3299-304. (PMID: 10866324) ; Cell. 2007 Dec 28;131(7):1248-59. (PMID: 18160036) ; Science. 1995 Oct 6;270(5233):50-1. (PMID: 7569949) ; Yeast. 1998 Jan 30;14(2):115-32. (PMID: 9483801) ; Genes Dev. 2001 Sep 1;15(17):2177-96. (PMID: 11544175) ; J Biol Chem. 2000 Nov 24;275(47):37011-20. (PMID: 10973982) Grant Information: R01 GM026259 United States GM NIGMS NIH HHS; R37 GM026259 United States GM NIGMS NIH HHS; GM26259 United States GM NIGMS NIH HHS Substance Nomenclature: 0 (Cell Cycle Proteins) ; 0 (DNA-Binding Proteins) ; 0 (Intracellular Signaling Peptides and Proteins) ; 0 (Saccharomyces cerevisiae Proteins) ; 0 (Tumor Suppressor Proteins) ; 0 (XRS2 protein, S cerevisiae) ; EC 2.7.11.1 (ATM protein, human) ; EC 2.7.11.1 (Ataxia Telangiectasia Mutated Proteins) ; EC 2.7.11.1 (Protein Serine-Threonine Kinases) ; EC 2.7.11.1 (TEL1 protein, S cerevisiae) ; EC 6.5.1.- (DNA Repair Enzymes) Entry Date(s): Date Created: 20080716 Date Completed: 20080929 Latest Revision: 20211203 Update Code: 20231215 PubMed Central ID: PMC2546937

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

A novel Tel1/ATM N-terminal motif, TAN, is essential for telomere length maintenance and a DNA damage response.