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Unlike the heterodimeric poly(A) polymerase (PAP) of vaccinia virus (VACV), the PAP from the Amsacta moorei entomopoxvirus, AMEV, is potentially derived from three subunits: a single large and two small subunits (AMV060 and AMV115). The VACV small subunit serves as a 2'-O-methyltransferase, a processivity factor for mRNA polyadenylation, and a transcription elongation factor. We wished to determine the structure-function relationships of the three putative AMEV PAP subunits. We show that AMV060 is expressed as an early gene persisting throughout infection, whereas AMV115 is expressed late. We demonstrate that AMV060 exhibits 2'-O-methyltransferase activity but the gene is not essential for virus growth. Absence of the AMV060 protein has no effect on the length of the poly(A) tails present in mRNA. No physical association was found between any of the putative AMEV PAP subunits. We therefore propose that mRNA polyadenylation does not require interactions between these three proteins.

Titel:	An Amsacta moorei entomopoxvirus ortholog of the poly(A) polymerase small subunit exhibits methyltransferase activity and is non-essential for virus growth.
Autor/in / Beteiligte Person:	Becker, MN ; Todd, TM ; Moyer, RW
Zeitschrift:	Virology, Jg. 375 (2008-06-05), Heft 2, S. 624-36
Veröffentlichung:	New York, Academic Press., 2008
Medientyp:	academicJournal
ISSN:	0042-6822 (print)
DOI:	10.1016/j.virol.2008.02.023
Schlagwort:	Amino Acid Sequence Animals Cell Line DNA-Directed RNA Polymerases Genes, Viral Larva virology Molecular Sequence Data Moths virology Polynucleotide Adenylyltransferase chemistry Protein Subunits genetics Sequence Alignment Viral Proteins chemistry Virus Replication Entomopoxvirinae physiology Methyltransferases metabolism Polynucleotide Adenylyltransferase metabolism Protein Subunits metabolism Viral Proteins metabolism
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, N.I.H., Extramural Language: English [Virology] 2008 Jun 05; Vol. 375 (2), pp. 624-36. <i>Date of Electronic Publication: </i>2008 Mar 26. MeSH Terms: Entomopoxvirinae / physiology ; Methyltransferases / metabolism ; Polynucleotide Adenylyltransferase / metabolism ; Protein Subunits / metabolism ; Viral Proteins / *metabolism ; Amino Acid Sequence ; Animals ; Cell Line ; DNA-Directed RNA Polymerases ; Genes, Viral ; Larva / virology ; Molecular Sequence Data ; Moths / virology ; Polynucleotide Adenylyltransferase / chemistry ; Protein Subunits / genetics ; Sequence Alignment ; Viral Proteins / chemistry ; Virus Replication References: Virology. 2006 Mar 30;347(1):88-99. (PMID: 16378629) ; Virol J. 2006 Mar 01;3:10. (PMID: 16509968) ; Mol Cell. 2006 May 5;22(3):339-49. (PMID: 16678106) ; Virology. 2002 Sep 15;301(1):64-80. (PMID: 12359447) ; Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2897-901. (PMID: 1313572) ; Virology. 2000 Aug 15;274(1):120-39. (PMID: 10936094) ; J Biol Chem. 1997 Sep 12;272(37):23292-302. (PMID: 9287339) ; Virology. 1995 Aug 20;211(2):462-73. (PMID: 7645250) ; Cell. 1991 Sep 20;66(6):1269-78. (PMID: 1670500) ; J Biol Chem. 1978 Nov 10;253(21):7692-7. (PMID: 701281) ; J Biol Chem. 1975 Jun 25;250(12):4722-9. (PMID: 237910) ; Cell. 1996 Apr 19;85(2):247-56. (PMID: 8612277) ; Virus Genes. 1999;19(1):23-31. (PMID: 10499447) ; Genes Dev. 1992 Aug;6(8):1575-86. (PMID: 1353739) ; J Virol. 2003 Jul;77(13):7590-600. (PMID: 12805459) ; J Gen Virol. 2004 Jan;85(Pt 1):105-117. (PMID: 14718625) ; Virology. 1998 Jul 5;246(2):253-65. (PMID: 9657944) ; J Virol. 1999 Jan;73(1):251-9. (PMID: 9847328) ; J Virol. 2004 Oct;78(19):10265-75. (PMID: 15367592) ; Nucleic Acids Res. 1985 Feb 11;13(3):985-98. (PMID: 2987815) ; J Insect Physiol. 2005 Feb;51(2):207-20. (PMID: 15749105) ; Nature. 1990 Nov 1;348(6296):91-2. (PMID: 2234068) ; J Virol. 1999 Jan;73(1):533-52. (PMID: 9847359) ; RNA. 1996 Jan;2(1):88-101. (PMID: 8846300) ; Virology. 2007 Feb 5;358(1):233-47. (PMID: 17005230) ; Virology. 2000 Apr 10;269(2):356-69. (PMID: 10753714) ; Virology. 2000 Apr 10;269(2):345-55. (PMID: 10753713) ; J Virol. 2006 Mar;80(5):2127-40. (PMID: 16474121) Grant Information: R01 AI046479 United States AI NIAID NIH HHS; R01 AI046479-05 United States AI NIAID NIH HHS; R01 AI 46479 United States AI NIAID NIH HHS Substance Nomenclature: 0 (Protein Subunits) ; 0 (Viral Proteins) ; EC 2.1.1.- (Methyltransferases) ; EC 2.7.7.19 (Polynucleotide Adenylyltransferase) ; EC 2.7.7.6 (DNA-Directed RNA Polymerases) Entry Date(s): Date Created: 20080328 Date Completed: 20080715 Latest Revision: 20211020 Update Code: 20240513 PubMed Central ID: PMC2478561

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An Amsacta moorei entomopoxvirus ortholog of the poly(A) polymerase small subunit exhibits methyltransferase activity and is non-essential for virus growth.