Einzeltreffer — DigiBib

Histone acetylation provides a switch between transcriptionally repressive and permissive chromatin. By regulating the chromatin structure at specific promoters, histone acetyltransferases (HATs) carry out important functions during differentiation and development of higher eukaryotes. HAT complexes are present in organisms as diverse as Saccharomyces cerevisiae, humans, and flies. For example, the well-studied yeast SAGA is related to three mammalian complexes. We previously identified Drosophila melanogaster orthologues of yeast SAGA components Ada2, Ada3, Spt3, and Tra1 and demonstrated that they associate with dGcn5 in a high-molecular-weight complex. To better understand the function of Drosophila SAGA (dSAGA), we sought to affinity purify and characterize this complex in more detail. A proteomic approach led to the identification of an orthologue of the yeast protein Ada1 and the novel protein encoded by CG4448, referred to as WDA (will decrease acetylation). Embryos lacking both alleles of the wda gene exhibited reduced levels of histone H3 acetylation and could not develop into adult flies. Our results point to a critical function of dSAGA and histone acetylation during Drosophila development.

Titel:	The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation.
Autor/in / Beteiligte Person:	Guelman, S ; Suganuma, T ; Florens, L ; Weake, V ; Swanson, SK ; Washburn, MP ; Abmayr, SM ; Workman, JL
Zeitschrift:	Molecular and cellular biology, Jg. 26 (2006-10-01), Heft 19, S. 7178-89
Veröffentlichung:	2023- : [Philadelphia] : Taylor & Francis ; <i>Original Publication</i>: [Washington, D.C.] : American Society for Microbiology, [c1981-, 2006
Medientyp:	academicJournal
ISSN:	0270-7306 (print)
DOI:	10.1128/MCB.00130-06
Schlagwort:	Acetylation Amino Acid Sequence Animals Chromatography, Affinity Conserved Sequence Drosophila Proteins chemistry Drosophila Proteins genetics Drosophila Proteins isolation & purification Drosophila melanogaster genetics Embryo, Nonmammalian cytology Gene Deletion Histone Acetyltransferases chemistry Histone Acetyltransferases isolation & purification Homozygote Multiprotein Complexes isolation & purification Multiprotein Complexes metabolism Protein Binding Protein Subunits chemistry Protein Subunits genetics Trans-Activators chemistry Trans-Activators metabolism Drosophila Proteins metabolism Drosophila melanogaster metabolism Genes, Essential genetics Genes, Insect genetics Histone Acetyltransferases metabolism Histones metabolism Protein Subunits metabolism Repetitive Sequences, Amino Acid
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article Language: English [Mol Cell Biol] 2006 Oct; Vol. 26 (19), pp. 7178-89. MeSH Terms: Repetitive Sequences, Amino Acid* ; Drosophila Proteins / metabolism ; Drosophila melanogaster / metabolism ; Genes, Essential / genetics ; Genes, Insect / genetics ; Histone Acetyltransferases / metabolism ; Histones / metabolism ; Protein Subunits / *metabolism ; Acetylation ; Amino Acid Sequence ; Animals ; Chromatography, Affinity ; Conserved Sequence ; Drosophila Proteins / chemistry ; Drosophila Proteins / genetics ; Drosophila Proteins / isolation & purification ; Drosophila melanogaster / genetics ; Embryo, Nonmammalian / cytology ; Gene Deletion ; Histone Acetyltransferases / chemistry ; Histone Acetyltransferases / isolation & purification ; Homozygote ; Multiprotein Complexes / isolation & purification ; Multiprotein Complexes / metabolism ; Protein Binding ; Protein Subunits / chemistry ; Protein Subunits / genetics ; Trans-Activators / chemistry ; Trans-Activators / metabolism References: Mol Cell Biol. 2000 Jan;20(1):312-8. (PMID: 10594033) ; Mol Cell Biol. 1999 Jan;19(1):855-63. (PMID: 9858608) ; Nature. 2000 Jan 6;403(6765):41-5. (PMID: 10638745) ; Gene. 2000 May 16;249(1-2):1-16. (PMID: 10831834) ; Microbiol Mol Biol Rev. 2000 Jun;64(2):435-59. (PMID: 10839822) ; Nature. 2000 Jun 8;405(6787):701-4. (PMID: 10864329) ; J Biol Chem. 2000 Aug 11;275(32):24928-34. (PMID: 10825174) ; Nat Genet. 2000 Oct;26(2):229-32. (PMID: 11017084) ; Nat Biotechnol. 2001 Feb;19(2):153-6. (PMID: 11175730) ; Mol Cell Biol. 2001 Mar;21(5):1841-53. (PMID: 11238921) ; Trends Biochem Sci. 2001 Apr;26(4):250-7. (PMID: 11295558) ; Genes Dev. 2001 Apr 15;15(8):1021-30. (PMID: 11316795) ; Science. 2001 Jun 22;292(5525):2333-7. (PMID: 11423663) ; Genes Dev. 2001 Jul 1;15(13):1619-24. (PMID: 11445536) ; Nat Struct Biol. 2001 Aug;8(8):695-700. (PMID: 11473260) ; Mol Cell Biol. 2001 Oct;21(20):6782-95. (PMID: 11564863) ; Mol Cell Biol. 2001 Nov;21(21):7331-44. (PMID: 11585915) ; Cell Mol Life Sci. 2001 Dec;58(14):2085-97. (PMID: 11814058) ; J Biol Chem. 2002 Mar 8;277(10):7989-95. (PMID: 11773077) ; Mol Cell Biol. 2002 Jul;22(13):4723-38. (PMID: 12052880) ; Mol Genet Genomics. 2002 Dec;268(4):425-33. (PMID: 12471440) ; Genome Biol. 2002;3(12):RESEARCH0079. (PMID: 12537568) ; Plant Cell. 2003 Mar;15(3):626-38. (PMID: 12615937) ; Curr Opin Cell Biol. 2003 Apr;15(2):172-83. (PMID: 12648673) ; Mol Cell Biol. 2003 May;23(9):3305-19. (PMID: 12697829) ; Trends Genet. 2003 Jun;19(6):321-9. (PMID: 12801725) ; Annu Rev Genomics Hum Genet. 2003;4:89-117. (PMID: 14527298) ; Cell. 2004 Jan 23;116(2):259-72. (PMID: 14744436) ; Mol Cell Biol. 2004 Apr;24(7):2932-43. (PMID: 15024081) ; Mol Cell. 2004 Jul 23;15(2):199-208. (PMID: 15260971) ; Mol Cell Biol. 2004 Aug;24(16):7249-59. (PMID: 15282323) ; Mol Cell Biol. 2004 Sep;24(18):8080-9. (PMID: 15340070) ; Biochim Biophys Acta. 1971 Aug 26;246(2):263-8. (PMID: 5167297) ; Cell. 1978 May;14(1):105-13. (PMID: 667927) ; Development. 1993 Jun;118(2):401-15. (PMID: 8223268) ; Mol Cell Biol. 1996 Feb;16(2):593-602. (PMID: 8552087) ; Biochemistry. 1996 Nov 5;35(44):13985-94. (PMID: 8909296) ; Mol Cell Biol. 1997 Jan;17(1):519-27. (PMID: 8972232) ; Genes Dev. 1997 Jul 1;11(13):1640-50. (PMID: 9224714) ; Proc Natl Acad Sci U S A. 1998 Jan 6;95(1):132-7. (PMID: 9419341) ; Cell. 1998 Jul 10;94(1):35-44. (PMID: 9674425) ; Cell. 1998 Jul 10;94(1):45-53. (PMID: 9674426) ; Cell. 1998 Jul 24;94(2):239-49. (PMID: 9695952) ; Nature. 1998 Jul 30;394(6692):498-502. (PMID: 9697775) ; Methods. 1998 Aug;15(4):315-21. (PMID: 9740719) ; Dev Biol. 1998 Oct 15;202(2):157-71. (PMID: 9769169) ; Mech Dev. 1998 Nov;78(1-2):113-8. (PMID: 9858703) ; Mol Cell Biol. 1999 Jan;19(1):86-98. (PMID: 9858534) ; Mol Cell. 1998 Dec;2(6):863-7. (PMID: 9885573) ; Trends Biochem Sci. 1999 May;24(5):181-5. (PMID: 10322433) ; J Biol Chem. 1999 Jun 25;274(26):18285-9. (PMID: 10373431) ; Mol Cell Biol. 2005 Feb;25(3):1173-82. (PMID: 15657442) ; Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8472-7. (PMID: 15932940) ; Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8478-82. (PMID: 15932941) ; Cell. 2005 Jun 17;121(6):859-72. (PMID: 15960974) ; Mol Cell Biol. 2005 Sep;25(18):8215-27. (PMID: 16135810) ; Mol Cell Biol. 2005 Sep;25(18):8228-38. (PMID: 16135811) ; Mol Cell Biol. 2006 Feb;26(3):871-82. (PMID: 16428443) ; Mol Cell Biol. 2000 Jan;20(1):340-51. (PMID: 10594036) Substance Nomenclature: 0 (Drosophila Proteins) ; 0 (Histones) ; 0 (Multiprotein Complexes) ; 0 (Protein Subunits) ; 0 (Trans-Activators) ; 0 (WDA protein, Drosophila) ; EC 2.3.1.48 (GCN5 protein, Drosophila) ; EC 2.3.1.48 (Histone Acetyltransferases) Entry Date(s): Date Created: 20060919 Date Completed: 20061024 Latest Revision: 20190613 Update Code: 20240513 PubMed Central ID: PMC1592886

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

The essential gene wda encodes a WD40 repeat subunit of Drosophila SAGA required for histone H3 acetylation.